Study sites
We conducted field surveys and experiments near Charleston, South Carolina (SC) (Fort Johnson: 32.751305°N, 79.90142°W; Stono River: 32.75253°N, 80.0076° W) and Savannah, Georgia (GA) (Priest’s Landing: 31.96012° N, 81.01223°W; Bull’s River: 31.97458°N, 80.92287°W) in the USA. The hydro- dynamic forces in southeastern estuaries generate high turbidity and fluid soft sediments which reduce light attenuation and thus create a habitat that is largely inhospitable to macrophyte attachment and persistence (Byers et al. 2012 and references therein). Gracilaria invaded SC and GA estuaries in the early 2000s (E. E. Sotka unpubl. data), and on intertidal mudflats where Diopatra worms are common, Gracilaria presently represents 90-99% of the total macroalgal biomass (Byers et al. 2012). The green alga Ulva sp. can be found in colder months and attached to oyster shells, wooden debris, the hard calcareous tubes of the soda-straw worm and only rarely on Diopatra tubes (Berke 2012; N. M. Kollars, E. E. Sotka & C. Plante pers. obs.). Other macroalgae present in the system include red algae that are epiphytic on Gracilaria (of the genera Polysiphonia and Ceramium; Berke 2012, C. E. Gerstenmaier and E. E. Sotka pers. obs.) and rarely, Gracilaria tikvahiae (Berke 2012, N. M. Kollars & E. E. Sotka pers. obs.). The non-native Gracilaria and native Diopatra are both rare within the salt marshes and oyster beds that fringe the upper-intertidal edge of these mudflats. We performed all laboratory experiments within the Grice Marine Laboratory (College of Charleston, SC, USA). See related reference for citations.

Tidal growth

We measured the growth of Gracilaria at 3 tidal heights of the Fort Johnson mudflat (‘high’, approx. +0.61 m MLLW; ‘mid’ approx. +0.09 m MLLW; and ‘low’ approx. -0.02 to -0.09 m MLLW) during the spring (March-April; n = 3 per tidal height), summer (July-August, n = 5 per tidal height), and fall (September- October, n = 8 per tidal height) of 2013. At each tidal height, we strung a 3.00 g (± 0.05, acceptable range of variation) blotted wet mass piece of Gracilaria (predominantly collected from the mid-intertidal at ~0.0 m MLLW) through the end of a 15 cm piece of a 0.76 cm diameter 3-strand rope, attached the rope to a 30 cm long × 0.76 cm diameter PVC-post, and drove the post into the sediment until the seaweed laid on the surface of the benthos (see photograph of a replicate before transplantation in Supplement 1A at http://www.int-res.com/articles/suppl/m545p135_supp/). During the spring assay, we enclosed the seaweed within flexible mesh bags (1.5 cm mesh size) constructed from wildlife fencing and zipties to protect the biomass from potential loss due to water flow. We observed few broken-off Gracilaria fragments present in the bags at the end of the spring assay, and therefore, we did not enclose the seaweed in the summer or fall assays. After 4-6 wk, we recovered and defaunated the seaweed and calculated change in wet mass as relative growth rate per week (hereafter RGR; Hoffmann & Poorter 2002).

To measure Gracilaria growth along a water-depth gradient of the subtidal zone, we weaved seaweed pieces of 3.00 g (± 0.05, acceptable range of variation) blotted wet mass through rope and attached the rope pieces (n = 5) at 2.5 m intervals along a 10 m rope strung vertically between a surface buoy and a cement block. We enclosed the seaweed within mesh bags (1.5 cm mesh size) as in the spring intertidal assay. We lowered the buoy-rope-block system onto the benthos at 9 replicate locations of ~10 m depth (at high tide) in Charleston Harbor within 7.3 km of the Fort Johnson site. With this design, the pieces of seaweed at the ‘0 m’ mark remained just below the surface of the water, regardless of fluctuations in tidal height. Because Charles ton Harbor has an average tidal range of ~2 m, the other 4 chosen depths fluctuated with the tidal cycle and the true depth ranges were estimated at 0.5-2.5, 3-5, 5.5-7.5, and 8-10 m. After 8 wk (February through April 2013), we recovered and defaunated the seaweed pieces, measured blotted wet mass, and calculated RGR as before.

Related Reference:
Kollars, N.M., J.E. Byers and E.E. Sotka (2016) Invasive decor: an association between a native decorator worm and a non-native seaweed can be mutualistic. Marine Ecology Progress Series (DOI: 10.3354/meps11602)

Related Datasets:
MEPS_2016: Fig.2A - survey
MEPS_2016: Fig.3 - growth rate and depth
MEPS_2016: Fig.4A - worm growth
MEPS_2016: Fig.4B - stable isotopes
MEPS_2016: Fig.5A - field expt 2012
MEPS_2016: Fig.5B - field expt 2013

Kollars_fig2B.csv