Abundance of ammonia monooxygenase subunit A (amoA) genes determined using TaqMan quantitative polymerase chain reaction (qPCR). Archaeal amoA genes were quantified using quantitative PCR using the assay described in Mosier and Francis 2011. Complete methods are described in Santoro et al., submitted.
Related references:
Mosier, A. C., and C. A. Francis. 2011. Determining the distribution of marine and coastal ammonia-oxidizing archaea and bacteria using a quantitative approach. Methods Enzymol. 486: 205-221. doi:10.1016/B978-0-12-381294-0.00009-2
Santoro, A.E., Saito, M.A., Goepfert, T.J., Lamborg, C.H., Dupont, C.L., G.R. DiTullio. Thaumarchaeal ecotype distributions across the equatorial Pacific and their potential roles in nitrification and flux attenuation. Submitted to Limnology & Oceanography, April 2016.
Santoro, A. E. (2016) qPCR data for archaeal amoA ecotypes; samples collected on METZYME cruise.. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 24 May 2016) Version Date 2016-05-24 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/647622 [access date]
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