Dataset: Single amplified genomes (SAGs) of microbial cells isolated from deep-sea hydrothermal vent 'Crab Spa', East Pacific Rise, Pacific Ocean from R/V Atlantis AT15-38 and AT26-10, 2008 and 2014 (Microbial Communities at Deep-Sea Vents project)

ValidatedRelease Date:2019-04-15Final no updates expectedDOI: 10.26008/1912/bco-dmo.660802.3Version 3 (2016-10-05)Dataset Type:experimental

Principal Investigator: Dr Ramunas Stepanauskas (Bigelow Laboratory for Ocean Sciences)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Program: Dimensions of Biodiversity (Dimensions of Biodiversity)

Program: Center for Dark Energy Biosphere Investigations (C-DEBI)

Project: An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents (Microbial Communities at Deep-Sea Vents)


Abstract

Single amplified genomes (SAGs) of microbial cells isolated from deep-sea hydrothermal vent 'Crab Spa', East Pacific Rise, Pacific Ocean from R/V Atlantis AT15-38 and AT26-10, 2008 and 2014 (Microbial Communities at Deep-Sea Vents project)

The accession links are not yet public. They will be archived at the Integrated Microbial Genomes (IMG), at the Joint Genome Inst. (JGI), US Dept. of Energy (DOE). Please contact the PI for further details.


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Methods

Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403–410. doi:10.1016/s0022-2836(05)80360-2
Methods

Pruesse, E., Quast, C., Knittel, K., Fuchs, B. M., Ludwig, W., Peplies, J., & Glockner, F. O. (2007). SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Research, 35(21), 7188–7196. doi:10.1093/nar/gkm864
Methods

Stepanauskas, R., & Sieracki, M. E. (2007). Matching phylogeny and metabolism in the uncultured marine bacteria, one cell at a time. Proceedings of the National Academy of Sciences, 104(21), 9052–9057. doi:10.1073/pnas.0700496104
Related Research

McNichol, J., Sylva, S. P., Thomas, F., Taylor, C. D., Sievert, S. M., & Seewald, J. S. (2016). Assessing microbial processes in deep-sea hydrothermal systems by incubation at in situ temperature and pressure. Deep Sea Research Part I: Oceanographic Research Papers, 115, 221–232. doi:10.1016/j.dsr.2016.06.011