Dataset: Grazing experiment 4: Carbon and Nitrogen

Name: Grazing experiment 4: Carbon and Nitrogen data for low-high pCO2 acclimated Rhodomonas sp. cultures (E Hux Response to pCO2 project)
Published: 2016-11-10
Keywords: biota, oceans

Cite This Dataset

Project:

Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)

Principal Investigator:

M Brady Olson (Western Washington University, WWU)

Co-Principal Investigator:

Dr Brooke Love (Western Washington University, WWU)

Suzanne Strom (Western Washington University, WWU)

Student:

Kelly Ann Still

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version Date:

2016-11-10

Restricted:

Release Date:

2017-12-31

Validated:

Yes

Current State:

Final no updates expected

Grazing experiment 4: Carbon and Nitrogen data for low-high pCO2 acclimated Rhodomonas sp. cultures (E Hux Response to pCO2 project)

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Description

Related Reference:
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO2 induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&context=wwuet

Methods & Sampling

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. Some of the culture removed was used to evaluate chemical parameters. Samples for particulate cellular carbon and nitrogen were taken by gently vacuum filtering 100 ml from each pCO2 treatment replicate onto 21 mm muffled glass fiber filters. After filtration, filters were removed and placed in tin boats. Samples and controls (media blanks, filter blanks, and capsule blanks) were placed in a drying oven for 24 hours at 60 C, after which time they were removed and placed in a desiccator until analysis. Tin boats containing the filters and controls were folded into pellets and then combusted using a Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer at the UC Davis Stable Isotope Facility.

This work took place at the Shannon Point Marine Center, Bellingham, Washington.

Data Processing Description

Picograms per cell for carbon and nitrogen were calculated based on standard curves and were then normalized to per cell based on cell counts for the sample day.

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- nd (no data) was entered into all blank cells

Data Files

File
expt4_CN.csv (Comma Separated Values (.csv), 2.01 KB) MD5:45e49f3d6efac4afc8d7030e7069dd19 Primary data file for dataset ID 664351

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0961229

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Lab_Olson_B		2011-03-31	WWU		data info

Instruments

Elemental Analyzer

Supplied Name: MICRO cube elemental analyzer

Supplied Description:

Measured carbon and nitrogen composition: Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer at the UC Davis Stable Isotope Facility.

Instrument Type

Generic Name: Elemental Analyzer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/

Generic Description:

Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material.

Isotope-ratio Mass Spectrometer

Supplied Name: PDZ Europa 20-20 isotope ratio mass spectrometer

Supplied Description:

Measured carbon and nitrogen composition: Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer at the UC Davis Stable Isotope Facility.

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
treatment_replicate	sample identifier: treatment replicate that names the sample and the day of semi-continuous culture or blank type	unitless	sample
C_ug	carbon in sample	micrograms (ug)	TOC
C_adjusted	carbon minus the media blank	micrograms (ug)	TOC
N_ug	nitrogen in sample	micrograms (ug)	TON
N_adjusted	nitrogen minus the media blank	micrograms (ug)	TON
cell_count	cells per ml in the daily sample	cells/milliliter	cell_concentration
ml_filtered	volume of sample filtered	milliliters	vol_filt
total_cells	total number of cell on the filter	cells	cell_concentration
C_ug_cell	carbon per cell	micrograms/cell (ug/cell)	TOC
N_ug_cell	nitrogen per cell	micrograms/cell (ug/cell)	TON
C_pg_cell	carbon per cell	picograms/cell (pg/cell)	TOC
N_pg_cell	nitrogen per cell	picograms/cell (pg/cell)	TON
C_to_N	ratio of carbon to nitrogen	dimensionless	C_to_N

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Database

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Dataset: Grazing experiment 4: Carbon and Nitrogen