| Contributors | Affiliation | Role |
|---|---|---|
| Place, Sean | Sonoma State University | Principal Investigator |
| Copley, Nancy | Woods Hole Oceanographic Institution (WHOI BCO-DMO) | BCO-DMO Data Manager |
Specimens of Trematomus bernacchii, Pagothenia borchgrevinki, and Trematomus newnesi were collected by hook and line near the Delbridge Islands in McMurdo Sound (77°40′S 166°25′E ) from October through December, 2011 and September through December, 2012. Once collected, fish were transported back to McMurdo Station in aerated coolers where they were acclimated for one week in a flow-through aquarium (2400-L) in ambient seawater (-1.5°C and ~430µatm CO2). After the initial acclimation period, fish were randomly placed into one of four flow-through experimental treatment tanks (1240-L each) in order to assess the response to increased temperature (4°C), increased pCO2 (1000µatm) or a combination of increased temperature and increased pCO2. The treatment tanks consisted of a tank held at ambient conditions (control treatment; -1°C and 430µatm), a low temperature + high pCO2 tank (-1°C and 1000µatm), a high temperature + low pCO2 tank (4°C and 430µatm), and a high temperature + high pCO2 tank (4°C and 1000µatm). Trematomus bernacchii, Pagothenia borchgrevinki were sampled after t= 7, 28, and 56 days of acclimation to the experimental conditions. Trematomus newnesi were sampled after t= 7, 28, and 42 days of acclimation. Four biological replicates from each treatment and time point were selected for cluster generation using the Illumina® TruSeq RNA Sample Prep v2 Hs Protocol and sequencing via an Illumina® HiSeq 2500 Rapid Run initialized for both paired-end 150bp reads and single-end 100bp reads.
BCO-DMO Processing Notes: A flat file table of the accession numbers with associated metadata and links to NCBI was created and served.
| File |
|---|
RNA_seq.csv (Comma Separated Values (.csv), 605 bytes) MD5:07d572ffcf9e911488a79762adcd24dc Primary data file for dataset ID 665853 |
| Parameter | Description | Units |
| project | internal project identifier | unitless |
| location | specimen collection site | unitless |
| lat | latitude; north is positive | decimal degrees |
| lon | longitude; east is positive | decimal degrees |
| species | species name | unitless |
| NCBI_accession | NCBI Bioproject accession number | unitless |
| accession_link | link to NCBI BioProject page | unitless |
| Dataset-specific Instrument Name | Illumina® HiSeq 2500 |
| Generic Instrument Name | Automated DNA Sequencer |
| Dataset-specific Description | For genetic sequencing |
| Generic Instrument Description | General term for a laboratory instrument used for deciphering the order of bases in a strand of DNA. Sanger sequencers detect fluorescence from different dyes that are used to identify the A, C, G, and T extension reactions. Contemporary or Pyrosequencer methods are based on detecting the activity of DNA polymerase (a DNA synthesizing enzyme) with another chemoluminescent enzyme. Essentially, the method allows sequencing of a single strand of DNA by synthesizing the complementary strand along it, one base pair at a time, and detecting which base was actually added at each step. |
| Website | |
| Platform | McMurdo Station |
| Start Date | 2014-08-14 |
| End Date | 2016-04-20 |
| Description | Polar fish studies |
Extracted from the NSF award abstract:
The proposed research will investigate the interacting and potentially synergistic influence of two oceanographic features - ocean acidification and the projected rise in mean sea surface temperature - on the performance of Notothenioids, the dominant fish of the Antarctic marine ecosystem. Understanding the joint effects of acidification and temperature rise on these fish is a vital component of predicting the resilience of coastal marine ecosystems. Notothenioids have repeatedly displayed a narrow window of physiological tolerances when subjected to abiotic stresses. Given that evolutionary adaptation may have led to finely-tuned traits with narrow physiological limits in these organisms, this system provides a unique opportunity to examine physiological trade-offs associated with acclimation to the multi-stressor environment expected from future atmospheric CO2 projections. Understanding these trade-offs will provide valuable insight into the capacity species have for responses to climate change via phenotypic plasticity. As an extension to functional measurements, this study will use evolutionary approaches to map variation in physiological responses onto the phylogeny of these fishes and the genetic diversity within species. These approaches offer insight into the historical constraints and future potential for evolutionary optimization.
Note: Funding for this project has transferred from award PLR-1040945 to PLR-1447291, coincident with Principal Investigator's affiliation change. Dr. Jeff Dudycha is a former project Co-PI associated with original award PLR-1040945.
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
| Funding Source | Award |
|---|---|
| NSF Antarctic Sciences (NSF ANT) |