Table of Contents

• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Parameters
• Instruments
• Deployments
• Project Information
• Funding

This dataset was published as Table 1 from Harder et al (2016). It contains collection information and GenBank COI accession links for sea spider of the genus specimens Pallenopsis from the Southern Ocean and around the southern tip of S. America.

Related Reference: Harder, A.M., K.M. Halanych and A.R. Mahon. Diversity and distribution within the sea spider genus Pallenopsis (Chelicerata: Pycnogonida) in the Western Antarctic as revealed by mitochondrial DNA. Polar Biol (2016) 39:677-688. DOI 10.1007/s00300-015-1823-8.

From Harder et al (2016):

Sample collection
Samples were collected in 2006 and 2012-2013 aboard the RVIB Nathaniel B. Palmer and the ASRV Laurence M. Gould. Pycnogonids were obtained using a Blake trawl or epibenthic sled, and subsequently identified to genus level prior to preservation and shipment in ~95 % ethanol. Individuals were identified to species level according to standard pycnogonid taxonomic procedures (Child 1995; Weis and Melzer 2012b; Weis et al. 2014). In total, 64 specimens belonging to the genus Pallenopsis were collected and identified from 21 sampling sites, ranging from 53 16'S to 76 59'S and from 140 26'E to 37 26'W (Fig. 1). Sampling site locality information, including depth (when available), and GenBank accession numbers for all sequences generated for use in our analyses are provided in Table 1.

Molecular techniques
A tissue sample was taken from each individual as a 1-cm piece from the first tibial segment, and DNA was extracted from these using a Qiagen DNeasy Blood & Tissue Kit (Qiagen Inc., Valencia, CA) per manufacturer’s instructions. A ~650 bp portion of the cytochrome c oxidase subunit I (COI) gene was amplified using LCO-1490 (Folmer et al. 1994) and HCOoutout (Prendini et al. 2005). Each PCR mixture consisted of 1x PCR buffer, 0.75 U Taq DNA polymerase (5 PRIME Inc., Gaithersburg, MD), 2.5 mM Mg^+2, 10 nmol of each dNTP, 1 ul of template DNA, 0.5 uM of each primer, and water to 25 ul. The PCR cycling program began with an incubation at 94C for 2 min, followed by 38 cycles of 94C for 20 s, 46C for 30 s, and 65 C for 80 s, and concluded with a final extension at 65C for 7 min. Successful amplification was confirmed by visualizing PCR products on a 1% agarose gel stained with ethidium bromide. Target DNA was gel extracted and purified using a Qiagen QIAquick Gel Extraction Kit (Qiagen Inc., Valencia, CA) according to the manufacturer’s recommendations. Bidirectional Sanger sequencing of amplicons was performed atHigh Throughput Genomics Center (Seattle, WA). Obtained sequences were assembled and screened using Sequencher version 5.29 sequence analysis software (Gene Codes Corporation, Ann Arbor, MI), and aligned using Clustal W v1.8 (Thompson et al. 1994) in BioEdit v7.2.5 (Hall 1999). COI sequences were translated into amino acid sequences to further screen for sequencing error, including checking for frameshift mutations and stop codons.

BCO-DMO Processing notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- added links to NCBI accession pages
- converted lat and lon to decimal degrees
- replaced '-' with nd (no data)
- replaced special characters with ascii characters

Extracted from the NSF award abstract:

The research will explore the genetics, diversity, and biogeography of Antarctic marine benthic invertebrates, seeking to overturn the widely accepted suggestion that benthic fauna do not constitute a large, panmictic population. The investigators will sample adults and larvae from undersampled regions of West Antarctica that, combined with existing samples, will provide significant coverage of the western hemisphere of the Southern Ocean. The objectives are: 1) To assess the degree of genetic connectivity (or isolation) of benthic invertebrate species in the Western Antarctic using high-resolution genetic markers. 2) To begin exploring planktonic larvae spatial and bathymetric distributions for benthic shelf invertebrates in the Bellinghausen, Amundsen and Ross Seas. 3) To continue to develop a Marine Antarctic Genetic Inventory (MAGI) that relates larval and adult forms via DNA barcoding.