Dataset: Rhodomonas CN

Name: Carbon and nitrogen content of Rhodomonas cells grown with varying CO2 levels for copepod experiments, 2015 (OA-Copepod_PreyQual project)
Published: 2017-01-18
Keywords: biota, oceans

Cite This Dataset

Project:

Impacts on copepod populations mediated by changes in prey quality (OA-Copepod_PreyQual)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Principal Investigator:

M Brady Olson (Western Washington University, WWU)

Co-Principal Investigator:

Julie E. Keister (University of Washington, UW)

Dr Brooke Love (Western Washington University, WWU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version Date:

2017-01-18

Restricted:

Release Date:

2018-01-01

Validated:

Yes

Current State:

Final no updates expected

Carbon and nitrogen content of Rhodomonas cells grown with varying CO2 levels for copepod experiments, 2015 (OA-Copepod_PreyQual project)

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Description

This dataset includes carbon and nitrogen content data from Rhodomonas used in copepod experiments with varying pCO2 levels, from 2015.

Methods & Sampling

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (800ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. Some of the culture removed was used to evaluate chemical parameters. Samples for particulate cellular carbon and nitrogen were taken by gently vacuum filtering 100 ml from each pCO2 treatment replicate onto 21 mm muffled glass fiber (GF/F) filters. After filtration, filters were removed and placed in tin boats. Samples and controls (media blanks, filter blanks and capsule blanks) were placed in a drying oven for 24 hours at 60 °C, after which time they were removed and placed in a desiccator until analysis. Tin boats containing the filters and controls were folded into pellets, and then combusted using a Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer at the UC Davis Stable Isotope Facility.

Data Processing Description

The data are unprocessed.

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- replaced spaces with underscore

Data Files

File
Rhodo_CN.csv (Comma Separated Values (.csv), 3.07 KB) MD5:f064a0699a338c62f823016d5c7797ce Primary data file for dataset ID 675242

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1220381
NSF Division of Ocean Sciences	OCE-1220664

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Lab_Olson_B		2011-03-31	WWU		data info

Instruments

CHN Elemental Analyzer

Supplied Name: Micro Cube elemental analyzer

Supplied Description:

Instrument Type

Generic Name: CHN Elemental Analyzer

Acronym: CHN_EA

Generic Description:

A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.

Isotope-ratio Mass Spectrometer

Supplied Name: PDZ Europa 20-20 isotope ratio mass spectrometer

Supplied Description:

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
temp	Temperature at which the Rhodomonas were grown	degrees Celsius	temp
treatment	CO2 level for growth conditions (ambient = 400; moderate = 800; high = 1200)	unitless	treatment
date	date of sample collection	unitless	date
TOC_ug	Total micrograms of Carbon in sample	micrograms	TOC
TON_ug	Total micrograms of Nitrogen in sample	micrograms	TON
C_per_cell_pg	picograms of Carbon per cell in sample	picograms	TOC
C_to_N	ratio of Carbon to Nitrogen in sample	dimensionless	C_to_N

Status message

Database

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Dataset: Rhodomonas CN