Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Parameters
• Instruments
• Deployments
• Project Information
• Funding

This dataset contains temperature, conductivity, turbidity, fluorometry, and meteorological data from the Underway Data Acquisition System (UDAS) on the R/V Point Sur cruise PS13-12 between 2013-06-27 to 2013-07-05.

The Underway Data Acquisition System (UDAS) on the R/V Point Sur consists of a suite of meteorological and sea surface sensors sampling at ~ 1 Hz, the entire duration the vessel is away from the dock. A constant flow of seawater (originating from an intake on the ship's seachest, just below the sea surface, ~ 2.5m) feeds the sensors and drains into a sink/drain in the Main Lab where it may be sampled by scientists or plumbed to science supplied equipment. The meteorological sensors are located on the mast above the Wheelhouse. All data is interfaced and collected on one computer located in the ET lab, via SCS, a data collection application developed by NOAA.

Acquisition description source (Moss Landing Marine Laboratories): https://marineops.mlml.calstate.edu/PS-UDAS (now broken as of 2017-08-30)

Cleaning steps performed by data contributor before being sent to BCO-DMO: 
- All daily csv files combined to master file. 
- "-999" = "no data" 
- All "NaN" turned to "-999" 
- combined Latitude & Longitude degrees, minutes, seconds into Decimal degrees 
-- Eliminated columns: 
-- Ashtech GPS coordinates (No long data recorded)
-- Rel_STBD_WD (no dynamic range, nearly all data ~270)
-- Rel STBD WS (no dynamic range, all data 0)
-- As in original excel sheet the following were omitted: SSC, SWDI, LWDI, PIR PSP, PIR dome C, PIR case C, PIR V therm, AVG Circ C, Battery V
- Eliminated the following data points and changed to -999
- Air temp F (3 values, 449, 454, 486)
-- Air temp C (3 values, 231, 234, 252)
-- Solar radiation (2 values, 13934, 1991)
-- SST (94 values recorded from 68 to -83)
- Salinity (8 values >40)
-- Xmiss% (325 values, % <0)
-- Fluoro (1 value <0)

BCO-DMO Data Manager Processing Notes:
* added a conventional header with dataset name, PI name, version date (can't use spaces, slashes, percent signs)
* modified parameter names to conform with BCO-DMO naming conventions
* -999 values changed to "nd" for no data so they are recognized in our system
* ISO timestamp added based on YMD, and HMS fields
* SST, Salinity, SST_TSAL rounded to three decimal places, lat/lon rounded to 5 decimal places

This study will examine the distribution of silica production among diatom species, using a novel combination of existing approaches, to evaluate the contributions of specific species or genera to total diatom silica production. Specific hypotheses regarding the distribution of silica production among species of diatoms will be tested by exploiting the strong contrasts in diatom community structure and silica production between a coastal upwelling system and in an oligotrophic subtropical gyre. Several lines of evidence support the idea that the diatoms responsible for the majority of silica production shifts from the most numerically abundant species in coastal systems to relatively rare, but very large, cells in offshore oligotrophic environments. This shift alters the role of diatoms in regional food webs and because many processes determining the role of phytoplankton groups in biogeochemical cycles are a function of cell size, such a shift has strong implications for regional differences in the contribution of diatoms to upper-ocean carbon cycling and the biological pump. 

This study also seeks to understand of the role of silicon limitation in regulating diatom silica production at the species level. Si limitation of silica production has been detected in every system examined to date, ranging from the high Si waters of the Antarctic, to coastal upwelling systems and the oligotrophic subtropical gyres. Field studies of Si limitation are rarely accompanied by examination of the species present. When studies do have taxonomic data the lack of information on the performance of individual species makes it impossible to allocate the measured rates among cells, potentially leading to erroneous conclusions about the contribution of specific diatom groups to community composite rates.

The project will test five hypotheses. Each is related to the general theme of using species-specific data to improve understanding of the factors regulating diatoms' role in marine food webs. By combining bulk measures of silica production using the radioisotope 32Si with quantitative measures of silicon deposition rates by individual cells using the fluorescent probe 2-(4-pyridyl)-5((4-(2-dimethylaminoethyl-aminocarbamoyl)-methoxy)phenyl)oxazole, or PDMPO, the following will be determined: species-specific diatom contributions to total community silica production, regional differences in the distribution of silica production among diatom species as a function of cell size, species-specific kinetic parameters governing the ability of species to compete for dissolved silicon, and whether dominance of a particular diatom group or species can be explained by knowledge of their capacity to utilize Si and their numerical abundance (as opposed to other factors such as grazing or limitation by other nutrients).