Dataset: Oikopleura dioica feeding behavior particle tracking

All experimental animals were obtained from the appendicularian culture facility at the Sars Centre for Marine Molecular Biology in Bergen, Norway on May 16, 2016. Immature day-6 Oikopleura dioica were filmed individually using a Sony 4K FDR-AX100 (HD resolution, 120 fps) mounted to a Nikon Eclipse E400 with a 10x objective using a Martin Microscope M99 Camcorder Adapter. Animals were filmed in a glass embryo dish filtering a suspension of 10 mm-diameter spheres and ellipsoids of similar width (L=22 +- 2.4 mm and W=7.8 +- 1.1 mm; n=5; mean +- SD).

For measurements of ellipsoidal particle orientation, the image stack was registered using the StackReg plugin with an Affine transform to account for the inflation and deflation of the feeding mesh. Images were then inverted and frames for trajectory analysis were color-coded in one of six channels using the Stack-to-Hyperstack tool. Stacks were Z-projected and the composite image was used to show trajectories. Measurements of ellipsoidal particle orientation were made using the straight line angle tool in ImageJ (41) and converted to be relative to the fluid flow.