Contributors | Affiliation | Role |
---|---|---|
Binder, Brian | University of Georgia (UGA) | Principal Investigator |
Ake, Hannah | Woods Hole Oceanographic Institution (WHOI BCO-DMO) | BCO-DMO Data Manager |
Prochlorococcus and Synechococcus cell counts in dilution experiment treatments.
BCO-DMO Data Processing Notes:
- removed rows that had all blank cells
- replaced all decimal points in column headers with underscores
- removed units from column names
File |
---|
dilutionexpercellcounts.csv (Comma Separated Values (.csv), 10.78 KB) MD5:97750ea595800c88d60c78a6775d65be Primary data file for dataset ID 716979 |
Parameter | Description | Units |
Cruise | R/V Cape Hatteras Cruise Designation | unitless |
Exper | Experiment Designation | unitless |
Bottle | Unique incubation bottle identifier | unitless |
Depth_Sample | Depth of source water | meters |
Depth_Incubate | Depth of incubation | meters |
Dilution | Fraction of unfiltered seawater in bottle | number |
Time | Sampling time; timepoints | unitless |
Pro | Prochlorococcus Cell Concentration | cells per milliliter |
Syn | Synechococcus Cell Concentration | cells per milliliter |
Count | Number of counts underlying cell concentrations | number |
Dataset-specific Instrument Name | Coulter-EPICS 753 flow cytometer |
Generic Instrument Name | Flow Cytometer |
Dataset-specific Description | Used to analyze preserved samples |
Generic Instrument Description | Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) |
Dataset-specific Instrument Name | Go-flo bottle |
Generic Instrument Name | GO-FLO Bottle |
Dataset-specific Description | Used to take seawater samples |
Generic Instrument Description | GO-FLO bottle cast used to collect water samples for pigment, nutrient, plankton, etc. The GO-FLO sampling bottle is specially designed to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths. |
Website | |
Platform | R/V Cape Hatteras |
Report | |
Start Date | 2009-05-20 |
End Date | 2009-06-02 |
Description | Project: Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Development and Application of a Reciprocal Transplant/Dilution Approach |
Website | |
Platform | R/V Cape Hatteras |
Report | |
Start Date | 2010-05-20 |
End Date | 2010-06-02 |
Description | Project: Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Development and Application of a Reciprocal Transplant/Dilution Approach |
The intellectual merit of the research is to extend our understanding of the biology and ecology of marine picophytoplankton, a group of microbes that are responsible for a large proportion of the total photosynthetic carbon fixation that occurs in the world's oceans. The importance of picophytoplankton as the dominant primary producers in open-ocean ecosystems is well-established. However, the factors that regulate the distribution and abundance of these populations remain poorly understood. The investigators will explore the dynamics of top-down (grazer-mediated) regulation of picophytoplankton populations in a specific context: the maintenance of summertime subsurface maxima in the pico-cyanobacterium Prochlorococcus (but not Synechococcus) in the Sargasso Sea. This phenomenon represents a relatively simple and predictable model system within which to test hypotheses about the regulation of oceanic picophytoplankton in general.
Recent results suggest that despite their abundance, Prochlorococcus in the subsurface maxi-mum are growing (and being grazed) rather slowly, as compared to the smaller population at the surface. In order to understand the factors responsible for this apparent paradox, this project will use a combination of field and laboratory studies to characterize and compare the interactions between Prochorococcus and its protozoan grazers at these two contrasting depths, and in relation to Synechococcus, which forms no such sub-surface maximum.
The broader impacts include training for graduate and undergraduate students. In addition, given the significance of picophytoplankton as primary producers at the base of oceanic microbial food webs, the results of this project should inform efforts to describe and model the broader oceanic ecosystem, and ultimately to understand its role in the global carbon cycle.
Funding Source | Award |
---|---|
NSF Division of Ocean Sciences (NSF OCE) |