Dataset: BrownOsenberg_2018- Fluorescein dye

Name: Concentration of Fluorescein dye after release over corals in the Moorea, French Polynesia (Vermetids_Corals project)
Published: 2017-10-05
Keywords: oceans

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Cite This Dataset

Spatial Extent: N:-17.47279 E:-149.78277 S:-17.48365 W:-149.84698

Project:

Spatial patterns of coral-vermetid interactions: short-term effects and long-term consequences (Vermetids_Corals)

Principal Investigator:

Anya L. Brown (University of Georgia, UGA)

Co-Principal Investigator:

Craig Osenberg (University of Georgia, UGA)

Contact:

Anya L. Brown (University of Georgia, UGA)

BCO-DMO Data Manager:

Mathew Biddle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2017-10-05

Version Date:

2017-10-05

Restricted:

Yes

Validated:

Concentration of Fluorescein dye after release over corals in the Moorea, French Polynesia (Vermetids_Corals project)

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Description

Belongs to set of datasets that evaluate how vermetid (Ceraesignum maximum) modify physical and chemical conditions above coral-algal interactions (i.e., light, flow and oxygen).

Related Datasets:

BrownOsenberg_2018- Fluorescein dye: https://www.bco-dmo.org/dataset/717831 (The current page)
BrownOsenberg_2018- FlDyeSurvey: https://www.bco-dmo.org/dataset/720777
BrownOsenberg_2018- InitO2_DBLthick: https://www.bco-dmo.org/dataset/720822
BrownOsenberg_2018- LightSensor: https://www.bco-dmo.org/dataset/720874
BrownOsenberg_2018- OxygenConcentrations: https://www.bco-dmo.org/dataset/720960

Methods & Sampling

Experiments and surveys were conducted in the field to determine how vermtetids affect light, flow and oxygen concentrations. Below are the methods for one of the field surveys on the effects of vermetids on flow over coral-algal interactions.

For each coral, algae or coral-algal interaction that was encountered covered by a mucus net, a syringe with a needle attached to it would be inserted under the net, and 2ml of 40mg/L of Fluorescein dye was inserted under the net. After 2s, 2ml of water was taken up by a rinsed syringe and placed in a vial to be checked later by a fluorometer. The net was then removed, and the after several seconds, dye was again released and re-sampled. This was completed on coral surfaces and coral-algal interfaces. Corals were massive Porites and algae were algal turf.

Materials: snorkel gear, dive slate, stopwatch, 40mg/L Fluorescein dye, syringe, needle, flourometer.

Location: Moorea, French Polynesia (-17.48 degrees S, -149.82 degrees W)

Data Processing Description

effect_difference column is the difference between net_presence-net_absence.

BCO-DMO Processing:

added conventional header with dataset name, PI name, version date
modified parameter names to conform with BCO-DMO naming conventions

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1130359

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Osenberg_et_al_Moorea		2003-05-19	Osenberg et al Moorea	Craig Osenberg (Principal Investigator)	data info

Instruments

Diving Mask and Snorkel

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Diving Mask and Snorkel

Generic Description:

A diving mask (also half mask, dive mask or scuba mask) is an item of diving equipment that allows underwater divers, including, scuba divers, free-divers, and snorkelers to see clearly underwater.

Snorkel: A breathing apparatus for swimmers and surface divers that allows swimming or continuous use of a face mask without lifting the head to breathe, consisting of a tube that curves out of the mouth and extends above the surface of the water.

Fluorometer

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Underwater Writing Slate

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Underwater Writing Slate

Generic Description:

Underwater writing slates and pencils are used to transport pre-dive plans underwater, to record facts whilst underwater and to aid communication with other divers.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Date	Date data collected in yyyymmdd format	unitless	date
Observer	data collector: Anya Brown (AB) or Julie Zill (JZ)	unitless	person
algae_presence	presence (Interaction) or absence (coral)	unitless	no_bcodmo_term
net_presence	Dye concentration in presence of mucus nets	unitless	treatment
net_absence	Dye concentration in absence of mucus nets	unitless	treatment
effect_difference	Difference between net_present and net_absent	unitless	treatment

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Database

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Dataset: BrownOsenberg_2018- Fluorescein dye