Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • BCO-DMO Processing Description
• Data Files
• Parameters
• Instruments
• Deployments
• Project Information
• Funding

This dataset includes holographic images from a LISST-Holo Submersible Digital Holographic Camera during an AUV Honey Badger deployment in the North Pacific gyre in 2015. I Image metadata, cell counts (e.g. number of Hemiaulus cells in aggregate form), and all raw holographic images are in the RAW.zip (12GB) file.

Software for further processing the raw holographic images provided here is available at the Sequoia Scientific, Inc. LISST-Holo page The following montage and depth images can be created for every raw image using the LISST-Holo software.

The LISST-Holo Submersible Digital Holographic Camera from Sequoia Scientific, Inc. was mounted on in a tow fish deployed on a 10 m tether behind the sub-body of the AUV Honey Badger. Holographic images were acquired in bursts every 6 hours.  During each burst, 15 images were taken in 5 seconds.

The software "Batch-Holo" was used to generate the biovolume from particles with an equivalent spherical diameter of 11.094-58.1 microns.  See "Parameters" section for more details.

Hemiaulus and Rhizosolenia cells were identified by hand. Each image was viewed using the "Holo_Detail" software and any Hemiaulus or Rhizosolenia cells were counted.

The manual and specifications for the LISST-Holo system, and software used in this dataset are available on the instrument page.  The software can be obtained from the Sequoia Scientific, Inc. LISST-Holo page.

The calculation of cells per liter was done using the following the following formula:

Data version 2018-01-05 replaces version 2017-10-31. The new version includes updated calculations of cells per liter. The previous version used a multiplication factor of 602 while the new version uses 538 (see Methods & Sampling section).

BCO-DMO Data Manager Processing Notes for version 2017-10-31:
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions
* 406 values of #DIV/0! caused by zero division removed. They empty cells display as "nd" for "no data" in the served dataset.
* added CTDTime_ISO from CTD timestamp column. The CTDTime_ISO is in format yyyy-mm-ddTHH:MMZ.
* latitude,longitude,QtrLat,QtrLon rounded to 5 decimal places
* added imagename column based on the deployment number "004" and the holo image number (with 4 place 0 padding)
* added embedded with link to a thumbnail .jpg of each holographic image. Note, the thumbnails are not holographic images themselves, and are only useful for display purposes.

BCO-DMO Data Manager Processing Notes for version 2018-01-05:
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions
* 406 values of #DIV/0! caused by zero division removed. They empty cells display as "nd" for "no data" in the served dataset.
* added CTDTime_ISO from CTD timestamp column. The CTDTime_ISO is in format yyyy-mm-ddTHH:MMZ.
* latitude,longitude,QtrLat,QtrLon rounded to 5 decimal places
* added imagename column based on the deployment number "004" and the holo image number (with 4 place 0 padding)
* added embedded with link to a thumbnail .jpg of each holographic image. Note, the thumbnails are not holographic images themselves, and are only useful for display purposes.

NSF award abstract:
Remote areas of the ocean are difficult to sample for short-lived or episodic features. This project will use a new sampling platform, the Wave Glider, and provide a continuous presence in the central North Pacific gyre. The six month duration of the mission will allow repeated sampling as well as spatial coverage previously unavailable. This mission will incorporate phytoplankton specific sensors as well as a set of optical sensors that will provide information on distribution, physiology and aggregation of a unique diatom-nitrogen fixing cyanobacterium symbiosis. When completed, this program will have generated the first data sets that follow these diatom blooms over extended periods in the region. Access to this instrumentation was facilitated by the PacX challenge, an international competition to produce high quality research from long-duration autonomous vehicles in the North and South Pacific Ocean. As a result of winning that competition, the principal investigator has been awarded the use of 6 months of the Honey Badger Wave Glider time in 2014. The Wave Glider is a wave-powered surface vessel capable of extended duration missions. In order to maximize this the principal investigator will outfit the glider with advanced sensors to quantify zones of intense diatom activity and aggregation along mesoscale features in the Pacific (Project MAGI: Mesoscale feature-AGregate Interactions).

Note: This project is funded by an NSF RAPID award.

The Honey Badger Team, image courtesy of Tracy Villareal

Honey Badger, image courtesy of Tracy Villareal