Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Parameters
• Instruments
• Deployments
• Project Information
• Funding

Porewater sulfate from samples collected by pushcore from Guaymas Basin hydrothermal sediments on cruise AT37-06 in December 2016.

Publications associated with this project are as follows:
Schutte, C.A., A. Teske, B.J. MacGregor, V. Salman-Carvalho, G. Lavik, and D. de Beer. Filamentous giant Beggiatoaceae from Guaymas Basin are capable of both denitrification and dissimilatory nitrate reduction to ammonium (DNRA). For submission.

Sampling and Analytical Methodology: Freshly collected sediment cores were sliced on the ship into 3 cm layers; porewater was obtained by gently centrifuging freshly collected sediment in 50 ml conical Falcon tubes for ca. 5 to 10 minutes until the sediment had settled; one Falcon tube produced ca. 8 to 10 ml of porewater. For porewater sulfate measurements, 1 ml subsamples of the overlying porewater were drawn into syringes and injected through 0.45 μm filters into screw cap Eppendorf vials, each acidified with 50 µl of 6N HCl, and then gently bubbled with nitrogen for 4 min to remove sulfide; the samples were then stored at 4ºC before shipping and analysis.

Geochemical Analyses: SO4 was analyzed on a Dionex ICS-1000 using a RFIC IonPac AS22 4 X 250mm column and a RFIC IonPac AG22 Guard column 4 X 50 mm. The IC was calibrated using a 5 point std curve and a minimum r^2 value of 0.999. The MDL measured at 0.10 ppm SO4. A check standard from a 1000 ppm was made fom a Dionex 7 anion standard to a concentration of 15 ppm. An external Standard from HACH 100 ppm SO4 was used to make a 20 ppm QC standard. In general, QCs were analyzed for 10% of the samples.

Data Processing: The porewater data of Guaymas Basin sand Sonora Margin piston cores were tabulated in Excel sheets.

Quality Control: Note that Sample_IDs "D7A_10X_CKS" are internal quality control standards (15.5 mg/L) and Sample_IDs "DI_SPK" are external quality control standards (20.0 mg/L).

BCO-DMO Processing: 
- modified parameter names (replaced spaces with underscores);
- replaced commas in Sample_ID column with semi-colons.

Description from NSF award abstract:
Hydrothermally active sediments in the Guaymas Basin are dominated by novel microbial communities that catalyze important biogeochemical processes in these seafloor ecosystems. This project will investigate genomic potential, physiological capabilities and biogeochemical roles of key uncultured organisms from Guaymas sediments, especially the high-temperature anaerobic methane oxidizers that occur specifically in hydrothermally active sediments (ANME-1Guaymas). The study will focus on their role in carbon transformations, but also explore their potential involvement in sulfur and nitrogen transformations. First-order research topics include quantifying anaerobic methane oxidation under high temperature,in situ concentrations of phosphorus and methane , and with alternate electron acceptors; sulfate and sulfur-dependent microbial pathways and isotopic signatures under these conditions; and nitrogen transformations in methane-oxidizing microbial communities, hydrothermal mats and sediments.

This integrated biogeochemical and microbiological research will explore the pathways of and environmental controls on the consumption and production of methane, other alkanes, inorganic carbon, organic acids and organic matter that fuel the Guaymas sedimentary microbial ecosystem. The hydrothermal sediments of Guaymas Basin provide a spatially compact, high-activity location for investigating novel modes of methane cycling and carbon assimilation into microbial biomass. In the case of anaerobic methane oxidation, the high temperature and pressure tolerance of Guaymas Basin methane-oxidizing microbial communities, and their potential to uncouple from the dominant electron acceptor sulfate, vastly increase the predicted subsurface habitat space and biogeochemical role for anaerobic microbial methanotrophy in global deep subsurface diagenesis. Further, microbial methane production and oxidation interlocks with syulfur and nitrogen transformations, which will be explored at the organism and process level in hydrothermal sediment microbial communities and mats of Guaymas Basin. In general, first-order research tasks (rate measurements, radiotracer incorporation studies, genomes, in situ microgradients) define the key microbial capabilities, pathways and processes that mediate chemical exchange between the subsurface hydrothermal/seeps and deep ocean waters.