Dataset: 515F-926R 16S rRNA gene sequencing accessions from seawater and sediment samples from R/V Falkor FK141109 and R/V Thompson TN309 from the Mariana and Kermadec trenches, 2014 (Mariana Perspectives project)

ValidatedFinal no updates expectedVersion (2017-12-13)Dataset Type:Cruise Results

Principal Investigator: Douglas Bartlett (University of California-San Diego Scripps)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)

Project: Patterns of Microbial Community Structure Within and Between Hadal Environments (Mariana Perspectives)

Cite Dataset

This dataset includes links to 515F-926R 16S rRNA gene sequencing accessions archived at NCBI and associated collection data from seawater and sediment samples from the Mariana and Kermadec trenches from April and November 2014.

For those accessions with a status of 'not yet available' at NCBI, they will be made available once the paper has been published, likely by mid-2018. Please check back for them then or contact the PI.

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Methods
Fuhrman JA, Comeau DE, Hagstrom A, Chan AM. Extraction from natural plankton microorganisms of DNA suitable for molecular biological studies. Appl Environ Microbiol. 1988;54: 1426-1429.
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Lever, M. A., Torti, A., Eickenbusch, P., Michaud, A. B., Šantl-Temkiv, T., & Jørgensen, B. B. (2015). A modular method for the extraction of DNA and RNA, and the separation of DNA pools from diverse environmental sample types. Frontiers in Microbiology, 6. doi:10.3389/fmicb.2015.00476
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Parada, A. E., Needham, D. M., & Fuhrman, J. A. (2016). Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. Environmental Microbiology, 18(5), 1403–1414. doi:10.1111/1462-2920.13023
Methods
Rusch, D. B., Halpern, A. L., Sutton, G., Heidelberg, K. B., Williamson, S., Yooseph, S., … Venter, J. C. (2007). The Sorcerer II Global Ocean Sampling Expedition: Northwest Atlantic through Eastern Tropical Pacific. PLoS Biology, 5(3), e77. doi:10.1371/journal.pbio.0050077
Methods
Tarn, J., Peoples, L. M., Hardy, K., Cameron, J., & Bartlett, D. H. (2016). Identification of Free-Living and Particle-Associated Microbial Communities Present in Hadal Regions of the Mariana Trench. Frontiers in Microbiology, 7. doi:10.3389/fmicb.2016.00665

Bartlett, D. (2017) 515F-926R 16S rRNA gene sequencing accessions from seawater and sediment samples from R/V Falkor FK141109 and R/V Thompson TN309 from the Mariana and Kermadec trenches, 2014 (Mariana Perspectives project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version ) Version Date 2017-12-13 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/720916 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation