11:I[76597,["4439","static/chunks/e2799680-28fe66354a53a27e.js","4035","static/chunks/4035-187f68ca241c9922.js","7648","static/chunks/7648-d4d7960e95f95a86.js","1519","static/chunks/1519-241ea71dbbd97a4d.js","9110","static/chunks/9110-d6c4c433ca4266da.js","5878","static/chunks/5878-6a906c6c6e5ebee4.js","9282","static/chunks/9282-cc3f04fbce522d1b.js","1550","static/chunks/1550-b36e7e0f3ebe978f.js","3401","static/chunks/3401-ee58bb219937dd8f.js","6770","static/chunks/app/dataset/%5Bid%5D/data/view/page-c9c121f6d2a6148a.js"],"default"] 12:T7d7,

Sampling and Analytical Methodology:

Seawater was collected in 12-L polyvinylchloride bottles affixed to a 24-bottle rosette

sampler, equipped with a Sea-Bird 911+ conductivity, temperature, and pressure profiler. Seawater samples were sequentially filtered using a peristaltic pump onto 25-mm diameter, 3-μm pore size polycarbonate membranes (Millipore IsoporeTM), followed by filtration through 25-mm diameter, 0.2-μm pore size polyethersulfone filters (Pall Supor®). After filtration, filters were placed in sterile 1.5-mL microcentrifuge tubes, immediately flash-frozen in liquid nitrogen, and stored at -80°C until analysis. Back in the shore-based laboratory, DNA from the 0.2-μm filter membranes was extracted and purified using the QIAGEN DNeasy Plant Mini Kit including a bead-beating step (with 0.1- and 0.5-mm beads) and Proteinase K (QIAGEN) for additional cell disruption and lysing. Extracts were eluted in 200 μL of nuclease-free PCR grade water and quantified using the Qubit® fluorometer. Triplicate PCR products were pooled, fragment size was determined using 1.5% agarose gel electrophoresis, and quantity was measured with the Qubit® 2.0 fluorometer with Qubit® dsDNA High Sensitivity Assay kit (Molecular Probes). From each sample, ~50 ng of PCR product was combined and purified using the UltraClean PCR Clean-Up Kit (MoBio).

Data Processing:

Sequences were merged using PEAR (Zhang et al.,