Dataset: Rhodomonas cell volume

Name: Rhodomonas cells volumes grown with varying CO2 levels for copepod experiments, 2015 (OA-Copepod_PreyQual project)
Published: 2018-01-25
Keywords: biota, oceans

Cite This Dataset

Temporal Extent: 2015-07-04 - 2015-08-09

Project:

Impacts on copepod populations mediated by changes in prey quality (OA-Copepod_PreyQual)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Principal Investigator:

M Brady Olson (Western Washington University, WWU)

Co-Principal Investigator:

Julie E. Keister (University of Washington, UW)

Brooke Love (Western Washington University, WWU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version Date:

2018-01-25

Restricted:

Validated:

Current State:

Preliminary and in progress

Rhodomonas cells volumes grown with varying CO2 levels for copepod experiments, 2015 (OA-Copepod_PreyQual project)

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Description

This dataset includes cell volume measurements from Rhodomonas grown at varying pCO2 levels, and fed to copepods, from 2015.

Methods & Sampling

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (800ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. Cells were counted live using a Z2 Coulter Particle Counter and cell volumes were recorded for the first five cells counted from each treatment each day.

Data Processing Description

The data are unprocessed.

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions

Data Files

File
Rhodo_cell_volume.csv (Comma Separated Values (.csv), 4.52 KB) MD5:1261f75b1e5b6cddf48068cddd201991 Primary data file for dataset ID 724639

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1220381
NSF Division of Ocean Sciences	OCE-1220664

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Lab_Olson_B		2011-03-31	WWU		data info

Instruments

Coulter Counter

Supplied Name: Z2 Coulter Particle Counter

Supplied Description:

Used to count cell culture samples

Instrument Type

Generic Name: Coulter Counter

Generic Description:

An apparatus for counting and sizing particles suspended in electrolytes. It is used for cells, bacteria, prokaryotic cells and virus particles. A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions.

from https://en.wikipedia.org/wiki/Coulter_counter

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
date	date sample was taken	unitless	date
CO2_treatment	CO2 level for growth conditions (ambient = 400; moderate = 800; high = 1200)	unitless	treatment
temp	Temperature at which the Rhodomonas were grown	degrees Celsius	temp
cell_volume	average volume of cells	cubic micrometers (um^3)	volume

Status message

Database

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Dataset: Rhodomonas cell volume