Dataset: Phillips_2011 - Experiment 2 Settlement Challenge 8

Name: Experiment 2: Settlement challenge experiment after 8 days post hatch in Moorea, French Polynesia from October, 2009 (Vermetids_Corals project)
Published: 2017-10-05
Keywords: oceans

Cite This Dataset

Spatial Extent: N:-17.47279 E:-149.78277 S:-17.48365 W:-149.84698

Temporal Extent: 2009-10-10 - 2009-10-18

Project:

Spatial patterns of coral-vermetid interactions: short-term effects and long-term consequences (Vermetids_Corals)

Principal Investigator:

Nicole Phillips (Victoria University of Wellington)

Contact:

Nicole Phillips (Victoria University of Wellington)

BCO-DMO Data Manager:

Mathew Biddle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2017-10-05

Version Date:

2017-10-05

Restricted:

Current State:

Preliminary and in progress

Experiment 2: Settlement challenge experiment after 8 days post hatch in Moorea, French Polynesia from October, 2009 (Vermetids_Corals project)

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Description

These data are from an experiment that test the nutritional strategies of Ceraesignum (Dendropoma) maximum larvae. For additional datasets see related files.

Related Datasets:

Phillips_2011 - Experiment 1 Larval Mortality: https://www.bco-dmo.org/dataset/725276
Phillips_2011 - Experiment 1 Larval Size: https://www.bco-dmo.org/dataset/725317
Phillips_2011 - Experiment 1 Settlement Challenge 10: https://www.bco-dmo.org/dataset/725335
Phillips_2011 - Experiment 1 SettlementChallenge18: https://www.bco-dmo.org/dataset/725392
Phillips_2011 - Experiment 2 Larval Mortality: https://www.bco-dmo.org/dataset/725880
Phillips_2011 - Experiment 2 Larval Size: https://www.bco-dmo.org/dataset/725943
Phillips_2011 - Experiment 2 Larval Velum Size: https://www.bco-dmo.org/dataset/725957
Phillips_2011 - Experiment 2 Settlement Challenge 6: https://www.bco-dmo.org/dataset/725973
Phillips_2011 - Experiment 2 Settlement Challenge 8: https://www.bco-dmo.org/dataset/726002 (Current page)

Methods & Sampling

In this experiment, larval growth and metamorposis was tested using different food levels.

Larvae hatched on October 10, 2009 and ~50 were distributed into each tubs on 500mL filtered sea water (FSW). Because the greatest metamorphic success was in the Isochrysis galbana treatment during experiment 1, only that species was used in experiment 2. Three food densities were created high food (4 x 104 cells mL-1 ), low food (4 x 103 cells mL-1 ) plus an Unfed treatment in which larvae were raised in FSW.

Larvae were placed in settlement challenges at 6- and 8-day post-hatch. Procedures were as in the first experiment, except that on each day 15 larvae from each replicate container within each food treatment were pooled, then redistributed into three replicate containers with coral rubble in 200 mL FSW (total N = 45 larvae from each food treatment). Also, mortality was so high in the 6-day challenge that the challenge was terminated after 2 days rather than 3

Data Processing Description

BCO-DMO Processing:

added conventional header with dataset name, PI name, version date
modified parameter names to conform with BCO-DMO naming conventions
empty values were replaced with 'nd' (no data).

Data Files

File
Phillips_2011_Expt2_SettlementChallenge8.csv (Comma Separated Values (.csv), 1.16 KB) MD5:014fcc8e0c0f50bb20147cbb70973dbb Primary data file for dataset ID 726002

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1130359

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Osenberg_et_al_Moorea		2003-05-19	Osenberg et al Moorea	Craig Osenberg (Principal Investigator)	data info

Instruments

Hemocytometer

Supplied Name: hemocytometer

Supplied Description:

Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment.

Instrument Type

Generic Name: Hemocytometer

Generic Description:

A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Larval_food_treatment	type of food given to larvae (Isochrysis galbana = Iso; Dunaliella tertiolecta = Dun; 1:1 ratio of Iso and Dun = Mixed)	unitless	treatment
Replicate_settlement_container	replicate tub	unitless	replicate
initial_number_larvae	initial number of larvae	unitless	count
day_1_number_live_larvae_with_velum_1	number of live larvae with velum day 1 after start of challenge	unitless	count
day_1_number_dead	number of dead larvae on day 1 after start of challenge	unitless	count
day_1_number_missing_1	number of larvae missing on day 1 after start of challenge	unitless	count
day_1_number_larvae_without_velum_1	number of larvae without velum on day 1 after start of challenge	unitless	count
day_1_number_metamorphosed	number of larve that metamorphosized on day 1 after start of challenge	unitless	count
day_1_number_live_larvae_with_velum_2	UNKNOWN	unitless	count
day_1_number_dead_2	UNKNOWN	unitless	count
day_1_number_missing_2	UNKNOWN	unitless	count
day_1_number_larvae_without_velum_2	UNKNOWN	unitless	count
day_1_number_metamorphosed_2	UNKNOWN	unitless	count
day_2_number_live_larvae_with_velum	number of live larvae with velum day 2 after start of challenge	unitless	count
day_2_number_dead	number of dead larvae on day 2 after start of challenge	unitless	count
day_2_number_missing	number of larvae missing on day 2 after start of challenge	unitless	count
day_2_number_larvae_without_velum	number of larvae without velum on day 2 after start of challenge	unitless	count
day_2_number_metamorphosed	number of larve that metamorphosized on day 2 after start of challenge	unitless	count
end_number_live_larvae_left_with_velum	number of live larvae end of challenge (all of the data for the two days are added together)	unitless	count
end_number_dead	number of dead larvae end of challenge (all of the data for the two days are added together)	unitless	count
end_number_missing	number of larvae missing end of challenge (all of the data for the two days are added together)	unitless	count
end_number_larvae_left_without_velum	number of larvae without velum end of challenge (all of the data for the two days are added together)	unitless	count
end_number_metamorphosed	number of larve that metamorphosized end of challenge (all of the data for the two days are added together)	unitless	count
end_percent_without_velum_plus_metamorphosed	Percent of larvae without velum and that metamorphosized at end of experiment (all of the data for the two days are added together)	unitless (percent)	no_bcodmo_term

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Database

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Dataset: Phillips_2011 - Experiment 2 Settlement Challenge 8