Dataset: Plocamium culture carbonate chemistry

Name: Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)
Published: 2018-02-07
Keywords: biota, oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.726664.1

Spatial Extent: N:34 E:-118 S:33 W:-119

Temporal Extent: 2014-06 - 2015-02

Project:

Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae (Seaweed OA Resilience)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Principal Investigator:

Janet E. Kubler (California State University Northridge, CSUN)

Co-Principal Investigator:

Steve Dudgeon (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-02-07

Restricted:

Release Date:

2019-06-03

Validated:

Yes

Current State:

Final no updates expected

Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)

Abstract:

Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2018-02-07	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.726664.1	2019-06-03

Description

This dataset includes carbonate chemistry measurements in the Plocamium.cartilagineum growth chambers.

Related Datasets:
Plocamium carbon nitrogen and stable isotopes: Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project)

Plocamium culture: seawater delta13C: Stable isotope ratio and concentration of carbon in seawater during Plocamium culture experiments, 2014-2015 (Seaweed OA Resilience project)

Plocamium cultures pH and temperature: Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015 (Seaweed OA Resilience project)

Plocamium exptl treatments summary: Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial (Seaweed OA Resilience project)

Plocamium growth and biomass: Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project)

Plocamium pigments: Photosynthetic pigment concentrations in Plocamium cartilagineum, trials 3-8, 2014-2015 (Seaweed OA Resilience project)

Plocamium: pH drift: Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project)

Rapid Light Curves_PAM: Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions

Methods & Sampling

Plocamium cartilagineum was collected from Santa Catalina Island and Leo Carollo, CA in June - Nov. 2014 and Jan. 2015.

Carbonate chemistry parameters were measured by sampling pH and total alkalinity (TA) of water samples. Seawater samples from each culture pot during all 7 trials were collected approximately every other day in 50 ml Falcon tubes to verify experimental treatment conditions. Water samples were measured usually within 1-2 hours of sample collection. pH was determined using the m-cresol indicator dye method in a spectrophotometer (Dickson et al. 2007). TA samples were analyzed by potentiometric titration coupled to a pH electrode calibrated using certified reference material (CRM) from the Dickson laboratory at Scripps Oceanographic Institute and the pH electrode calibrated using TRIS buffer (Dickson et al. 2007). TA and carbonate parameters were calculated from potentiometric titration data and spectrophotometric pH data.

Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.

See Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).

Data Processing Description

Carbonate chemistry parameters (CO2 concentration, CO2 partial pressure, CO2 fugacity, HCO3, CO3, DIC, Omega Aragonite, Omega Calcite) and total scale pH at in situ temperature were calculated using the seacarb package (V3.0.14) in R (Lavigne et al. 2011).

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- combined data from 8 separate trial files
- reduced precision to meet sampling precision methods

Data Files

File
carb_chem.csv (Comma Separated Values (.csv), 178.55 KB) MD5:7958d68cf2f3ba0cf77d59ec8e9b96bd Primary data file for dataset ID 726664

Supplemental Files

File

Table of culture conditions for each of the 8 trials
filename: Dudgeon_seaweed_trial_meta_2018-01.pdf

(Portable Document Format (.pdf), 429.04 KB)
MD5:5a4a9cbd3cb5092a419b4883c96979b8

Notes: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses. In each culture pot, pCO2 was set by the supply rate of CO2 in the corresponding mass-flow controlled gas mixing system to be within a target range of either near ambient, moderately elevated or highly elevated in each trial. The near-ambient range was narrower than the other target ranges (set points typically ~380 - 390 micro-atm) as it served as the control range in each trial. However, actual pCO2 in solution in each culture pot varied slightly from constant target values on a diurnal cycle associated with the metabolic activities of the algae contained within each pot. Unique average values of pCO2 in each culture pot based on different set values for each mass-flow controlled mixer within the qualitative ranges of ambient, moderate and highly increased pCO2 levels that were replicated in each trial of the experiment enabled a more powerful regression-type experimental design. With a regression type approach we could estimate the functional relationship between response variables and pCO2, which was not possible with a simple categorical treatment design.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1316198

Deployments

None available yet

Instruments

Automatic titrator

Supplied Name: Mettler Toledo T50

Supplied Description:

Used to measure total alkalinity; equipped with Rondolino automated titration stand.

Instrument Type

Generic Name: Automatic titrator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Generic Description:

Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

Multi Parameter Portable Meter

Supplied Name:

Supplied Description:

YSI 556 MPS (Trials 1-5) and Thermo Fisher Orion Star 329 (trials 6-8) used to measure salinity and temperature.

Instrument Type

Generic Name: Multi Parameter Portable Meter

Generic Description:

An analytical instrument that can measure multiple parameters, such as pH, EC, TDS, DO and temperature with one device and is portable or hand-held.

Spectrophotometer

Supplied Name: Shimadzu UV-2450 UV-visible spectrophotometer

Supplied Description:

Used to measure pH (at temperature 25 C)

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
trial	experimental trial identifier	unitless	exp_id
expt_day	days from beginning of trial when data were collected	day number	days
date	date of measurment formatted as yyyy-mm-dd	unitless	date
sample	Sample replicate ID in each pot	unitless	sample
pH25	pH in Total scale at 25 deg C	unitless	pH
sal_insitu	Salinity in situ	parts per thousand (ppt)	sal
temp_insitu	Temperature in situ	degrees Celsius	temp
pH_insitu	pH-Total scale in situ temperature	unitless	pH
CO2	Carbon dioxide concentration	??mol/kilogram	TCO2
pCO2	CO2 partial pressure	??atmospheres (atm)	pCO2
fCO2	CO2 fugacity	??atmospheres (atm)	fCO2
HCO3	Bicarbonate ion concentration	??mol/kilogram	bicarbonate
CO3	Carbonate ion concentration	??mol/kilogram	carbonate
DIC	Total carbon dissolved inorganic	??mol/kilogram	DIC
ALK	Total Alkalinity measured	??mol/kilogram	TALK
OmegaAragonite	Aragonite saturation state	unitless	OM_ar
OmegaCalcite	Calcite Saturation State	unitless	OM_ca