Dataset: Symbiodinium cultures isolated from the octocoral Antillogorgia bipinnata in the Florida Keys and processed at Coffroth lab at the University at Buffalo in 2008, 2013 and 2016 (Host Symbiont Temp Response project)

ValidatedFinal no updates expectedDOI: 10.1575/1912/bco-dmo.728215.1Version 1 (2018-02-16)Dataset Type:experimental

Principal Investigator: Mary Alice Coffroth (State University of New York at Buffalo)

Co-Principal Investigator: Casey terHorst (California State University Northridge)

BCO-DMO Data Manager: Mathew Biddle (Woods Hole Oceanographic Institution)


Project: RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs (Host Symbiont Temp Response)


Abstract

Symbiodinium cultures isolated from the octocoral Antillogorgia bipinnata in the Florida Keys and processed at Coffroth lab at the University at Buffalo in 2008, 2013 and 2016.

Symbiodinium cultures isolated from adult colonies of the octocoral Antillogorgia bipinnata in 2008, 2013 and 2016, grown at either 26 or 30 degrees C.

Cultures initially isolated from Antillogorgia bipinnata colonies collected in the lower keys in the vicinity of Looe Key (24 32.973'N 81 22.849'W) in 2008, the middle keys near Tennessee Reef (24 45.150'N  81 45.275'W) in 2013 and the upper keys at Pickles Reef (24 59.016'N 80 24.832'W) and Elbow Reef (25 07.956'N 80 15.810'W and 25 07.925'N 80 15.717'W).  Culture have been maintained in the Coffroth lab, University at Buffalo.


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Methods

Guillard, R. R. L., & Ryther, J. H. (1962). STUDIES OF MARINE PLANKTONIC DIATOMS: I. CYCLOTELLA NANA HUSTEDT, AND DETONULA CONFERVACEA (CLEVE) GRAN. Canadian Journal of Microbiology, 8(2), 229–239. doi:10.1139/m62-029
Methods

Lajeunesse, T. C., Parkinson, J. E., & Reimer, J. D. (2012). A genetics-based description of Symbiodinium minutum sp. nov. and S. psygmophilum sp. nov. (Dinophyceae), two dinoflagellates symbiotic with cnidaria. Journal of Phycology, 48(6), 1380–1391. doi:10.1111/j.1529-8817.2012.01217.x
Methods

Parkinson, J. E., Coffroth, M. A., & LaJeunesse, T. C. (2015). New species of Clade B Symbiodinium (Dinophyceae) from the greater Caribbean belong to different functional guilds: S. aenigmaticum sp. nov., S. antillogorgium sp. nov., S. endomadracis sp. nov., and S. pseudominutum sp. nov. Journal of Phycology, 51(5), 850–858. doi:10.1111/jpy.12340
Methods

Santos, S. R., Gutierrez-Rodriguez, C., & Coffroth, M. A. (2003). Phylogenetic identification of symbiotic dinoflagellates via length heteroplasmy in Domain V of chloroplast Large Subunit (cp23S)-Ribosomal DNA Sequences. Marine Biotechnology, 5(2), 130–140. doi:10.1007/s10126-002-0076-z
Methods

Santos, S. R., Taylor, D. J., & Coffroth, M. A. (2001). GENETIC COMPARISONS OF FRESHLY ISOLATED VERSUS CULTURED SYMBIOTIC DINOFLAGELLATES: IMPLICATIONS FOR EXTRAPOLATING TO THE INTACT SYMBIOSIS. Journal of Phycology, 37(5), 900–912. doi:10.1046/j.1529-8817.2001.00194.x