Dataset: Ulva: Chl a

Name: Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project)
Published: 2018-03-22
Keywords: biota, oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.732438.1

Spatial Extent: N:34 E:-118 S:33 W:-119

Temporal Extent: 2015-05-11 - 2015-07-27

Project:

Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae (Seaweed OA Resilience)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Principal Investigator:

Janet E. Kubler (California State University Northridge, CSUN)

Co-Principal Investigator:

Steve Dudgeon (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-03-22

Restricted:

Release Date:

2019-06-03

Validated:

Yes

Current State:

Final no updates expected

Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project)

Abstract:

This dataset reports the chlorophyll a concentration per unit biomass of Ulva lactuca grown in closed culture pots at varying pCO2 levels.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2018-03-22	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.732438.1	2019-06-05

Description

This dataset reports the chlorophyll a concentration per unit biomass of Ulva lactuca grown in closed culture pots at varying pCO2 levels.

Related Datasets:
Ulva: Carbonate chemistry pCO2: Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project)
Ulva: CHN and stable isotopes: Stable isotope ratios and mass of carbon and nitrogen in Ulva cells under ocean acidification conditions (Seaweed OA Resilience project)
Ulva: Growth: Growth rates of Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
Ulva: pH and temperature time-series: Time-series at 10 minute sampling interval of pH and temperature in Ulva culture pots (Seaweed OA Resilience project)
Ulva: pH Drift: Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project)
Ulva: Photosynthesis and respiration: Rates of photosynthesis and respiration by Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
Ulva: seawater delta13C: Stable isotope ratio and concentration of carbon in seawater from Ulva OA experiments (Seaweed OA Resilience project)

Methods & Sampling

Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at 15° C under saturating illumination of ~550 µmoles photons/m^2/s on a 12:12 L:D cycle. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining nitrogen, oxygen and carbon dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being nitrogen.

Chlorophyll a was extracted from Ulva tissue following the procedure described in Duncan and Harrison (1982). Chlorophyll a concentrations were measured using a Shimadzu UV-2450 UV-visible spectrophotometer.

Data Processing Description

BCO-DMO Processing Notes:
- added a conventional header with dataset name and description, PI names, version date
- modified parameter names to conform with BCO-DMO naming conventions

Data Files

File
Chl_a_Ulva.csv (Comma Separated Values (.csv), 6.06 KB) MD5:13302458522a7c50790cb4247dfd48f0 Primary data file for dataset ID 732438

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1316198

Deployments

None available yet

Instruments

UV Spectrophotometer-Shimadzu

Supplied Name: Shimadzu UV-2450 UV-visible spectrophotometer

Supplied Description:

Used to measure chlorophyll a concentration.

Instrument Type

Generic Name: UV Spectrophotometer-Shimadzu

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Label	Trial number-pot number.frozen tissue replicate	unitless	sample
pCO2_avg	Average pCO2 partial pressure in seawater tanks	microatmospheres (µatm)	pCO2
pCO2_sd	Variability of pCO2 partial pressure - standard deviation	microatmospheres (µatm)	pCO2
Fresh_Wt	Fresh weight of tissue sample prior to deep freeze	grams	weight
DMSO_A665	Absorbance of light by ground Ulva tissue at 665nm in 1.25 ml of an 80% DMSO solution in water	unitless	absorbance
Methanol_A668	Absorbance of light by ground Ulva tissue at 668nm in 6 ml methanol	unitless	absorbance
Methanol_A635	Absorbance of light by ground Ulva tissue at 635nm in 6 ml methanol	unitless	absorbance
DMSO_ext	Chlorophyll a extracted in DMSO	milligram/liter (mg/L)	chl_a
Meth_ext	Chlorophyll a in pooled extracts of 12 ml of methanol	milligram/liter (mg/L)	chl_a
Total_chl_mg	Total chlorophyll a extracted from ground tissue	milligrams (mg)	chl_tot
chl_a	Chlorophyll a concentration per unit biomass	milligrams/gram (mg/g)	chl_a

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