Dataset: Ulva: CHN and stable isotopes

Name: Stable isotope ratios and mass of carbon and nitrogen in Ulva cells under ocean acidification conditions (Seaweed OA Resilience project)
Published: 2018-03-26
Keywords: biota, oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.732564.1

Spatial Extent: N:34 E:-118 S:33 W:-119

Temporal Extent: 2015-05-11 - 2015-07-27

Project:

Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae (Seaweed OA Resilience)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Principal Investigator:

Janet E. Kubler (California State University Northridge, CSUN)

Co-Principal Investigator:

Steve Dudgeon (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-03-26

Restricted:

Release Date:

2019-06-03

Validated:

Yes

Current State:

Final no updates expected

Stable isotope ratios and mass of carbon and nitrogen in Ulva cells under ocean acidification conditions (Seaweed OA Resilience project)

Abstract:

This dataset includes carbon and nitrogen content and stable isotope values from Ulva lactuca tissue grown at 15° C and various CO2 levels, from May 2015 through July 2015.

Expand/Collapse All

Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2018-03-26	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.732564.1	2019-06-05

Description

This dataset includes carbon and nitrogen content and stable isotope values from Ulva lactuca tissue grown at 15° C and various CO2 levels, from May 2015 through July 2015.

Related Datasets:
Ulva: Carbonate chemistry pCO2: Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project)
Ulva: Chl a: Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project)
Ulva: Growth: Growth rates of Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
Ulva: pH and temperature time-series: Time-series at 10 minute sampling interval of pH and temperature in Ulva culture pots (Seaweed OA Resilience project)
Ulva: pH Drift: Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project)
Ulva: Photosynthesis and respiration: Rates of photosynthesis and respiration by Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
Ulva: seawater delta13C: Stable isotope ratio and concentration of carbon in seawater from Ulva OA experiments (Seaweed OA Resilience project)

Methods & Sampling

Ulva was collected prior to each trial in May, June and July 2015 near Malibu, CA (34°04'12"N 118°56'69"W)

Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at 15C under saturating illumination of ~550 µmoles photons/m^2/s. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.

Data Processing Description

BCO-DMO Processing Notes:
- added a conventional header with dataset name and description, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions

Data Files

File
Ulva_CHN_iso.csv (Comma Separated Values (.csv), 4.88 KB) MD5:20798a547ba4c2785f60d548fb62291b Primary data file for dataset ID 732564

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1316198

Deployments

None available yet

Instruments

Isotope-ratio Mass Spectrometer

Supplied Name:

Supplied Description:

Instrument located at the University of California, Davis Stable Isotope Facility (UCD-SIF) and used to measure isotope carbon and nitrogen isotopes.

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

scale

Supplied Name: Analytical balance (Mettler Toledo XP205)

Supplied Description:

Used to weigh dried plant tissue samples.

Instrument Type

Generic Name: scale

Acronym: balance

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/

Generic Description:

An instrument used to measure weight or mass.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Sample_ID	Trial number: time=0 or end (E); pot number; replicate	unitless	sample
pCO2_avg	Average pCO2 partial pressure in seawater tanks	microatmospheres (µatm)	pCO2
pCO2_sd	Variability of pCO2 partial pressure - standard deviation	microatmospheres (µatm)	pCO2
d13C	Isotopic composition of delta 13C of plant relative to Pee Dee Belemnite (PDB)	parts per thousand (ppt)	d13C_bio
C_mass_sample	Mass of carbon in sample	micrograms	TOC
d15N	isotopic composition of delta 15N of plant relative to air	parts per thousand (ppt)	d15N_bio
N_mass_sample	Mass of nitrogen in sample	micrograms	TON
C_N	Ratio (by mass) of Carbon to Nitrogen	unitless	C_to_N
mass_plant	Mass of plant tissue sample	milligrams	mass_dry