Dataset: 57Fe Wall Loss Experiment
Data Citation:
Breitbart, M., Buck, K., Bonnain, C., Caprara, S. (2018) 57Fe Wall Loss Experiment data collected as part of a method development study investigating the precipitation and wall loss of labeled 57Fe when added to M9 Minimal Media. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-04-05 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.732864.1 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.
DOI:10.1575/1912/bco-dmo.732864.1
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Temporal Extent: 2017-11-13
Project:
EAGER: Iron-Virus Interactions in the Ocean
(Fe-Virus)
Principal Investigator:
Mya Breitbart (University of South Florida, USF)
Kristen Nicolle Buck (University of South Florida, USF)
Co-Principal Investigator:
Chelsea Bonnain (University of South Florida, USF)
Salvatore Caprara (University of South Florida, USF)
BCO-DMO Data Manager:
Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Version:
1
Version Date:
2018-04-05
Restricted:
No
Validated:
Yes
Current State:
Final no updates expected
57Fe Wall Loss Experiment data collected as part of a method development study investigating the precipitation and wall loss of labeled 57Fe when added to M9 Minimal Media
Abstract:
This data was collected as part of a method development study investigating the precipitation and wall loss of labeled ⁵⁷Fe when added to M9 Minimal Media, which is used to grow the model bacterial species Escherichia coli. The bulk media was prepared with the same components and a portion was treated with Chelex-100 to remove metals, while the other portion remained un-chelexed. Half of each treatment was spiked with labeled ⁵⁷Fe and either 0.2 μm or 0.02 μm filtered for comparison of the dissolved and soluble fractions. The ⁵⁷Fe content was monitored over four time points for one week in a shaking incubator, under the same conditions used to culture E. coli for labeling experiments.