Dataset: 57Fe Wall Loss Experiment data collected as part of a method development study investigating the precipitation and wall loss of labeled 57Fe when added to M9 Minimal Media

ValidatedFinal no updates expectedDOI: 10.1575/1912/bco-dmo.732864.1Version 1 (2018-04-05)Dataset Type:experimental

Principal Investigator: Mya Breitbart (University of South Florida)

Principal Investigator: Kristen Nicolle Buck (University of South Florida)

Co-Principal Investigator: Chelsea Bonnain (University of South Florida)

Co-Principal Investigator: Salvatore Caprara (University of South Florida)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Project: EAGER: Iron-Virus Interactions in the Ocean (Fe-Virus)


Abstract

This data was collected as part of a method development study investigating the precipitation and wall loss of labeled ⁵⁷Fe when added to M9 Minimal Media, which is used to grow the model bacterial species Escherichia coli. The bulk media was prepared with the same components and a portion was treated with Chelex-100 to remove metals, while the other portion remained un-chelexed. Half of each treatment was spiked with labeled ⁵⁷Fe and either 0.2 μm or 0.02 μm filtered for comparison of the disso...

Show more

This data was collected as part of a method development study investigating the precipitation and wall loss of labeled ⁵⁷Fe when added to M9 Minimal Media, which is used to grow the model bacterial species Escherichia coli. The bulk media was prepared with the same components and a portion was treated with Chelex-100 to remove metals, while the other portion remained un-chelexed. Half of each treatment was spiked with labeled ⁵⁷Fe and either 0.2 μm or 0.02 μm filtered for comparison of the dissolved and soluble fractions. The ⁵⁷Fe content was monitored over four time points for one week in a shaking incubator, under the same conditions used to culture E. coli for labeling experiments.


Related Datasets

No Related Datasets

Related Publications

Methods

Gledhill, M. (2012). The organic complexation of iron in the marine environment: a review. Frontiers in Microbiology, 3. doi:10.3389/fmicb.2012.00069
Methods

Kutter, E., & Sulakvelidze, A. (2004). Bacteriophages: biology and applications. CRC Press.
Methods

Mellett, T., Brown, M.T., Chappell, P.D., Duckham, C., Fitzsimmons, J.N., Till, C.P., Sherrell, R.M., Maldonado, M.T., and Buck, K.N. (2017). The biogeochemical cycling of iron, copper, nickel, cadmium, manganese, cobalt, lead, and scandium in a California Current experimental study. Limnology and Oceanography, 63(S1), S425–S447. doi:10.1002/lno.10751
Methods

Pai, S.-C., Whung, P.-Y., & Lai, R.-L. (1988). Pre-concentration efficiency of chelex-100 resin for heavy metals in seawater. Analytica Chimica Acta, 211, 257–270. doi:10.1016/s0003-2670(00)83685-5
Methods

Shrivastava, A., & Gupta, V. (2011). Methods for the determination of limit of detection and limit of quantitation of the analytical methods. Chronicles of Young Scientists, 2(1), 21. doi:10.4103/2229-5186.79345