Dataset: OA and temp expt. - water chemistry

Name: Measured and calculated water chemistry parameters throughout a 93-day acidification and warming experiment
Published: 2018-05-09
Keywords: oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.735629.1

Spatial Extent: N:16.1899 E:-88.2614 S:16.1167 W:-88.5728

Temporal Extent: 2015-06-01 - 2015-12-30

Project:

Investigating the influence of thermal history on coral growth response to recent and predicted end-of-century ocean warming across a cascade of ecological scales (Thermal History and Coral Growth)

Principal Investigator:

Karl D. Castillo (University of North Carolina at Chapel Hill, UNC-Chapel Hill)

Student:

Colleen Bove (University of North Carolina at Chapel Hill, UNC-Chapel Hill)

Contact:

Colleen Bove (University of North Carolina at Chapel Hill, UNC-Chapel Hill)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-05-09

Restricted:

Release Date:

2019-02-11

Validated:

Yes

Current State:

Final no updates expected

Measured and calculated water chemistry parameters throughout a 93-day acidification and warming experiment

Abstract:

This water chemistry data set includes water temperature, salinity, and pH of all 24 experimental aquaria measured every other day throughout the 93-day ocean acidification and warming experiment. Additionally, the data include the measured (temperature, salinity, total alkalinity, and dissolved inorganic carbon) and calculated (Dissolved CO2, carbonate ion concentration, bicarbonate ion concentration], pCO2, aragonite saturation state, and pH) carbonate parameters from water samples taken every 10 days during the experiment. These data were used to determine the experimental conditions of each treatment that the corals were exposed to.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2018-05-09	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.735629.1	2019-12-09

Description

Related datasets:
OA and temp experiment - coral growth: https://www.bco-dmo.org/dataset/735583
OA and temp experiment - survival: https://www.bco-dmo.org/dataset/735609

Methods & Sampling

Temperature, salinity, and pH measurements were conducted every other day throughout the experimental period. Temperature was measured using a high precision partial-immersion glass thermometer (precision ±0.3%; accuracy ±0.4%). Salinity (±SD) was measured using a YSI 3200 conductivity meter and maintained at 31.7 (±0.2), with slight natural seasonal variation as expected in Massachusetts Bay waters. A pH probe calibrated with 7.00 and 10.01 NBS buffers kept at experimental temperatures was used to measure pH in each tank. Water samples were taken from each tank every ten days around 13:00 Eastern Time using 250 mL ground-glass-stoppered borosilicate glass bottles for analysis of total alkalinity (TA) and dissolved inorganic carbon (DIC). TA and DIC were measured via coulometry and closed-cell potentiometric Gran titration, respectively calibrated with certified Dickson DIC/TA standards. Dissolved CO2 ([CO2 (SW)]), carbonate ion concentration [CO32–], bicarbonate ion concentration [HCO3–], pCO2, aragonite saturation state, and pH of the seawater from all treatments were calculated from measured temperature, salinity, DIC, and TA using CO2SYS (Pierrot et al, 2006) with Roy et al. (1993) carbonic acid constants K1 and K2 and NBS (mol kg–1 H2O) pH scale.

CO2SYS v2.1 was used to calculate carbonate parameters. http://cdiac.ess-dive.lbl.gov/ftp/co2sys/

Data Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- reduced decimal precision of 'sal' from 8 to 3
- reformatted date from d-Mon-yy to yyyy-mm-dd

Data Files

File
Bove_chem.csv (Comma Separated Values (.csv), 19.29 KB) MD5:82c6c976dcc5efe8ab0411a5b51453b5 Primary data file for dataset ID 735629

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1459522

Deployments

None available yet

Instruments

CO2 Coulometer

Supplied Name: UIC 5400

Supplied Description:

Total alkalinity (TA) and dissolved inorganic carbon (DIC) were analyzed via coulometry (UIC 5400) and via closed-cell potentiometric Gran titration, respectively (Marianda, VINDTA 3C) calibrated with certified Dickson DIC/TA standards (Scripps Institution of Oceanography; San Diego, California, USA).

Instrument Type

Generic Name: CO2 Coulometer

Acronym: CO2 coulometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12

Generic Description:

A CO2 coulometer semi-automatically controls the sample handling and extraction of CO2 from seawater samples. Samples are acidified and the CO2 gas is bubbled into a titration cell where CO2 is converted to hydroxyethylcarbonic acid which is then automatically titrated with a coulometrically-generated base to a colorimetric endpoint.

Conductivity Meter

Supplied Name: YSI 3200 conductivity meter with a 1.0 cm-1 cell (Yellow Springs, Ohio, USA).

Supplied Description:

Used to measure salinity.

Instrument Type

Generic Name: Conductivity Meter

Generic Description:

Conductivity Meter - An electrical conductivity meter (EC meter) measures the electrical conductivity in a solution. Commonly used in hydroponics, aquaculture and freshwater systems to monitor the amount of nutrients, salts or impurities in the water.

MARIANDA VINDTA 3C total inorganic carbon and titration alkalinity analyser

Supplied Name: Marianda, VINDTA 3C

Supplied Description:

Used to measure dissolved inorganic carbon (DIC). Calibrated with certified Dickson DIC/TA standards (Scripps Institution of Oceanography; San Diego, California, USA).

Instrument Type

Generic Name: MARIANDA VINDTA 3C total inorganic carbon and titration alkalinity analyser

Acronym: inorganic carbon and alkalinity analyser

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0481/

Generic Description:

The Versatile INstrument for the Determination of Total inorganic carbon and titration Alkalinity (VINDTA) 3C is a laboratory alkalinity titration system combined with an extraction unit for coulometric titration, which simultaneously determines the alkalinity and dissolved inorganic carbon content of a sample. The sample transport is performed with peristaltic pumps and acid is added to the sample using a membrane pump. No pressurizing system is required and only one gas supply (nitrogen or dry and CO2-free air) is necessary. The system uses a Metrohm Titrino 719S, an ORION-Ross pH electrode and a Metrohm reference electrode. The burette, the pipette and the analysis cell have a water jacket around them. Precision is typically +/- 1 umol/kg for TA and/or DIC in open ocean water.

pH Sensor

Supplied Name: accuFet Solid-State pH probe (Fisher Scientific Waltham, Massachusetts, USA)

Supplied Description:

The pH probe was calibrated with 7.00 and 10.01 NBS buffers which were kept at experimental temperatures; used to measure pH.

Instrument Type

Generic Name: pH Sensor

Generic Description:

An instrument that measures the hydrogen ion activity in solutions.

The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).

Thermometer

Supplied Name: high precision partial-immersion glass thermometer

Supplied Description:

Thermometer precision ±0.3%; accuracy ±0.4%; used to measure temperature.

Instrument Type

Generic Name: Thermometer

Generic Description:

A device designed to measure temperature.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
date	Date of sample collection formatted as yyyy-mm-dd	unitless	date
treatment	Experimental treatment fragment was in: first number represents target pCO2 value; second is the temperature treatment	unitless	treatment
pCO2	Measured partial pressure of carbon dioxide in tank	micro-atmospheres	pCO2
temp	Measured temperature in tank	degrees Celsius	temp
sal	Measured salinity in tank	practical salinity units (psu)	sal
pH	Measured pH in tank; NBS scale	unitless	pH
alk	Calculated total alkalinity (TA)	millimol/kilogram (mmol kg-1)	TALK
DIC	Measured dissolved inorganic carbon (DIC)	millimol/kilogram (mmol kg-1)	DIC
pH_cal	Calculated pH (using CO2SYS); NBS scale	unitless	pH
HCO3_cal	Calculated concentration of bicarbonate ion ([HCO3]-) (using CO2SYS)	millimol/kilogram (mmol kg-1)	bicarbonate
CO2_cal	Calculated concentration of carbon dioxide (CO2) (using CO2SYS)	millimol/kilogram (mmol kg-1)	TCO2
CO3_cal	Calculated concentration of carbonate ion ([CO3]2-) (using CO2SYS)	millimol/kilogram (mmol kg-1)	carbonate
sat_cal	Calculated aragonite saturation state (using CO2SYS)	unitless	OM_ar