Dataset: Symbiodinium genotype responses to temperature

Name: Traits of three Symbiodinium genotypes measured at ambient and elevated temperatures
Published: 2018-05-23
Keywords: climate change, coral reefs, evolutionary rescue, genetic variation, mutualism, natural selection, Symbiodinium, symbionts, biota, oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.738212.1

Spatial Extent: N:25.1326 E:-80.26195 S:24.54955 W:-81.75458

Temporal Extent: 2015-06-01 - 2015-07-31

Project:

RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs (Host Symbiont Temp Response)

Principal Investigator:

Casey terHorst (California State University Northridge, CSUN)

Co-Principal Investigator:

Mary Alice Coffroth (State University of New York at Buffalo, SUNY Buffalo)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-05-23

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Traits of three Symbiodinium genotypes measured at ambient and elevated temperatures

Abstract:

Traits of three genotypes measured at ambient and elevated temperatures. Replicates of each Symbiodinium genotype were grown in ambient (26 degrees) and elevated (30 degrees) temperatures, after which were measured physiological parameters, including the number of cells, quantum yield, variable fluorescence, and chlorophyll content.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2018-05-23	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.738212.1	2019-10-02

Description

This dataset includes the abundance, quantum yield, fluorescence, and chlorophyll values from three Symbiodinium genotypes that were grown at two temperatures, 26 and 30 degrees C.

Methods & Sampling

Replicates of each Symbiodinium genotype were grown in ambient (26 degrees) and elevated (30 degrees) temperatures, after which were measured physiological parameters, including the number of cells, quantum yield, variable fluorescence, and chlorophyll content.

Data Processing Description

BCO-DMO Processing notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- reduced decimal precision of NumberCells to 0

Data Files

File
genotypes_temp.csv (Comma Separated Values (.csv), 1.78 KB) MD5:bf8e0a23b2fb3e9b7b8e8c7c36c9d6e6 Primary data file for dataset ID 738212

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1559105

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Coffroth_2016		2016-08-25	SUNY-Buffalo	Mary Alice Coffroth (Principal Investigator)	data info

Instruments

Fluorometer

Supplied Name: Trilogy Laboratory Fluorometer

Supplied Description:

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Genotype	Genetic idenetifier	unitless	sample
Temp	Temperature	degrees Celsius	temp
NumberCells	Number of cells per mL	number cells/milliliter (#/mL)	cell_concentration
QY	Quantum Yield	unitless ratio	Fv_Fm
Vfl	Variable Fluorescence	unitless	Fv_Fm
Chla	Total Chlorophyll per sample	Relative Fluorescence Units	chl_a
Chlapercell	Chlorophyll per cell	Relative Fluorescence Units	chl_a
Replicate	Replicate identifier, designated as 'a' through 'e'.	unitless	replicate