Dataset: Chesapeake Bay: Nitrous Oxide

Name: Nitrous oxide concentrations from CTD bottle samples collected during R/V Hugh R. Sharp cruises HRS1316 and HRS1317 in Chesapeake Bay from August to September of 2013
Published: 2018-06-15
Keywords: oceans

Cite This Dataset

Spatial Extent: N:38.5576 E:-76.3095 S:38.3677 W:-76.5387

Temporal Extent: 2013-08-26 - 2013-09-17

Project:

Collaborative Research: New Approaches to New Production (N-SPOT)

Gene content, gene expression, and physiology in mesopelagic ammonia-oxidizing archaea (AmoA Archaea)

Principal Investigator:

Alyson E. Santoro (University of California-Santa Barbara, UCSB)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-06-15

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Nitrous oxide concentrations from CTD bottle samples collected during R/V Hugh R. Sharp cruises HRS1316 and HRS1317 in Chesapeake Bay from August to September of 2013

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Description

Related Datasets (also generated from samples during cruises HRS1316 and HRS1317):
* Chesapeake Bay: Ammonia oxidation rates https://www.bco-dmo.org/dataset/738599
* Chesapeake Bay: N2 to Ar ratio https://www.bco-dmo.org/dataset/738604
* Chesapeake Bay: Nitrate https://www.bco-dmo.org/dataset/738609
* Chesapeake Bay: Nitrite https://www.bco-dmo.org/dataset/738614
* Chesapeake Bay: Ammonium https://www.bco-dmo.org/dataset/738619

The results paper for these data has been submitted to the journal Estuaries and Coasts (Laperriere et al., submitted).

Methods & Sampling

Water samples were collected using a 12 x 10 L Niskin bottle rosette sampler equipped with a conductivity, temperature, and pressure instrument package (SBE9, Sea-Bird Electronics, Bellevue, Washington, U.S.A.) and a sensor for dissolved oxygen (SBE43, Sea-Bird).

N2O samples were collected in duplicate in 160 mL serum vials. The tubing was placed at the bottom of each container and water overflowed by approximately five volumes. The samples were preserved using 100 μL of a saturated mercuric chloride solution. The serum vials were sealed using butyl septa and aluminum crimp tops, and stored at room temperature, which was cooler than sampling temperature, until analysis.

A 30 mL ultra-high purity N2 headspace was added to each sample using a syringe with a vent needle inserted in the septa to drain sample water. Each headspace was over-pressurized with an additional 2.5 mL of N2 to avoid atmospheric contamination upon headspace sample removal. The headspace was equilibrated with the underlying seawater by gentle shaking at room temperature for at least 2 hours.

N2O concentrations were measured using a headspace equilibration method and analyzed on a Shimadzu GC-14B Gas Chromatograph (GC) equipped with a Porapak-Q packed column and an electron capture detector (ECD) (Elkins 1980). N2O concentrations were calculated according to Walter et al. 2006.

Data Processing Description

BCO-DMO Data Manager Processing Notes:
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions
* blank values in this dataset are displayed as "nd" for "no data." nd is the default missing data identifier in the BCO-DMO system.
* removed spaces between minutes and seconds
* removed trailing and leading spaces of values
* removed occasional space after decimal place in original longitude decimal degree format
* added timestamp in UTC (ISO format)
* changed time format from HH:MM:00 AM/PM to 24 hour time to be consistent with other datasets.

Data Files

File
N2O.csv (Comma Separated Values (.csv), 23.28 KB) MD5:418db5ccd281dd33c3c2754df00b5274 Primary data file for dataset ID 738594

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1260006
NSF Division of Ocean Sciences	OCE-1437310
NSF Division of Ocean Sciences	OCE-1740538

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
HRS1316	1301	2013-08-25	R/V Hugh R. Sharp	Michael R. Roman (Chief Scientist)	data info
HRS1317	1302	2013-09-12	R/V Hugh R. Sharp	Michael R. Roman (Chief Scientist)	data info

Instruments

CTD Sea-Bird 9

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: CTD Sea-Bird 9

Acronym: CTD SBE 9

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/130/

Generic Description:

The Sea-Bird SBE 9 is a type of CTD instrument package. The SBE 9 is the Underwater Unit and is most often combined with the SBE 11 Deck Unit (for real-time readout using conductive wire) when deployed from a research vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorometer, altimeter, etc.). Note that in most cases, it is more accurate to specify SBE 911 than SBE 9 since it is likely a SBE 11 deck unit was used. more information from Sea-Bird Electronics

Gas Chromatograph

Supplied Name: Shimadzu GC-14B Gas Chromatograph

Supplied Description:

Shimadzu GC-14B Gas Chromatograph (GC) equipped with a Porapak-Q packed column and an electron capture detector (ECD)

Instrument Type

Generic Name: Gas Chromatograph

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/

Generic Description:

Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)

Niskin bottle

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
station	Station number	unitless	station
date	Date of sample collection (local EDT)	unitless	date
time	Time of sample collection (local EDT)	untiless	time
lat	Latitude (N)	degrees decimal minutes	lat
lon	Longitude (W)	degrees decimal minutes	lon
depth	Depth	meters	depth
n2o	Nitrous oxide concentration	nanomoles per liter (nmol/L)	N2O
replicate	Replicate number	unitless	replicate
ISO_DateTime_UTC	ISO timestamp based on the ISO 8601:2004(E) standard in format YYYY-mm-ddTHH:MMZ (UTC)	unitless	ISO_DateTime_UTC
lat_dd	Latitude	decimal degrees	lat
lon_dd	Longitude	decimal degrees	lon

Status message

Database

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Dataset: Chesapeake Bay: Nitrous Oxide