Dataset: A. pisonii: egg fatty acids

Name: Fatty acid concentration extracted from A. pisonii eggs from three habitats along the Florida eastern coast, 2016
Published: 2018-07-16
Keywords: biota, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.741219.1

Spatial Extent: N:30.1325 E:-80.28611 S:27.43 W:-81.38556

Temporal Extent: 2016-06 - 2016-10

Project:

Linking Variation in Metabolic Processes as a Key to Prediction (Variation in Metabolic Processes)

Principal Investigator:

Dr Blaine D. Griffen (University of South Carolina)

Co-Principal Investigator:

Zachary J. Cannizzo (University of South Carolina)

Contact:

Zachary J. Cannizzo

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-07-16

Restricted:

Release Date:

2020-07-16

Validated:

Yes

Current State:

Final no updates expected

Fatty acid concentration extracted from A. pisonii eggs from three habitats along the Florida eastern coast, 2016

Abstract:

This dataset includes concentrations of fatty acids from eggs of mangrove tree crabs, A. pisonii, from three habitats: mangrove, saltmarsh and mangrove collected along the eastern Florida coast in 2016.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2018-07-16	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.741219.1	2020-07-16

Description

Methods & Sampling

We collected 10 ovigerous females with stage-1 non-eyed eggs by hand and from each habitat (mangrove, salt marsh, dock) during the week preceding the full moon of each of five consecutive months throughout the A. pisonii reproductive season. Crabs were immediately placed on dry ice and stored at -80oC until dissection at which time the size and gut-width were determined and the whole egg clutch was carefully removed from the pleopods. The egg clutch was then freeze-fried and stored at -80oC until analysis.

Lipids were extracted from eggs using a modified Folch extraction and the fatty acids were then methylated. We then analyzed the methylated fatty acids via gas chromatography-mass spectrometry using an Agilent 6890 GC 5975 Mass Spectrometer fitted with a Restek 30m FAMEWAX column. The concentration of each FA was then determined from a diluton curve derived from a Supelco 37 Fatty Acid mix. The FA peak areas were first normalized to recovery standard then concentrations were determined using the regression curves of the external standards. This concentration was then corrected for the volume of the sample to obtain the mass of the fatty acid extracted from the egg.

Gut-width:carapace-width ratio was calculated as the ratio of the size of the gut of the crab and the size of the crab.

The mass of Omega-3 Fatty Acids was determined by combining the masses of Alpha Linoleic Acid, Eicosatrienoic Acid, Eicosapentaenoic Acid, and Docosahexaenoic Acid.

The mass of Omega-6 Fatty Acids was determined by combining the masses of cis-Linoleic Acid, trans-Linoleic Acid, Eicosadienoic Acid, Dihomo-Gamma-Linolenic Acid, Arachidonic Acid, and Docosahexaenoic Acid.

The mass of the highly-unsaturated Fatty Acids was determined by combining the masses of Eicosapentaenoic Acid, Arachidonic Acid, and Docosahexaenoic Acid.

The mass of odd-numbered Fatty Acids was determined by combining the masses of C15:0 fatty acid, C15:1 fatty acid, C17:0 fatty acid, and C17:1 fatty acid.

The mass of each fatty acid extracted from the eggs was divided by the mass of the eggs from which fatty acids were extracted to obtain the concentration of that fatty acid in the eggs as the proportion of the egg mass.

Locations, Florida East Coast:
Round Island Park: 27o33'33"N 80o19'53"W
Pepper Park: 27o29'42'N 80o18'12"W
North Causeway Park: 27o28'28"N 80o19'12"W
Oslo Road: 27o35'14"N 80o21'55"W
Anastasia State Park: 29o52'40"N 81o16'32"W
Guana-Tolomato-Matanzas NERR: 30o0'49"N 81o20'42"W
Vilano Inlet: 29o55'16"N 81o17'57"W
Palm Valley/Nocatee Canoe Launch: 30o07'57"N 81o23'08"W
St. Augustine Yacht Club: 29o53'09"N 81o17'08"W
Boating Club Road: 29o56'34"N 81o18'31"W

Data Processing Description

BCO-DMO Processing:
- reduced decimal precision of: GW_CW from 9 to 3 places

Data Files

File
Apisonii_fatty_acids.csv (Comma Separated Values (.csv), 59.53 KB) MD5:319f20ab547f7dd81e7ef54f7215ff48 Primary data file for dataset ID 741219

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1129166

Deployments

None available yet

Instruments

Mass Spectrometer

Supplied Name: Agilent 6890 GC 5975 Mass Spectrometer

Supplied Description:

Instrument Type

Generic Name: Mass Spectrometer

Acronym: Mass Spec

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.

scale

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: scale

Acronym: balance

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/

Generic Description:

An instrument used to measure weight or mass.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Sample	individual ID number given to each crab	unitless	sample
Habitat	The habitat where the crab was collected and observed.	unitless	site_descrip
Site	Site of observation/collection. RI= Round Island Park; PP=Pepper Park; NC=North Causeway Park; Oslo=Oslo Road; ANA=Anastasia State Park; GTM= Guana-Tolomato-Matanzas NERR; VM=Vilano Inlet; PV= Palm Valley/Nocatee Canoe Launch; YC=St. Augustine Yacht Club; BC=Boating Club Road	unitless	site
Year	Year of collection	unitless	year
Month	Number of month (1-12) that crab was collected	unitless	month
CW	Size of crab; measured as carapace-width	millimeters	width
GW_CW	gut-width:carapace-width ratio	unitless	no_bcodmo_term
Egg_ug	Mass of the eggs from which the fatty acids were extracted.	micrograms	lipids_biota
C14_0	Mass of C14:0 fatty acid extracted from eggs.	micrograms	lipids_biota
C14_1	Mass of C14:1 fatty acid extracted from eggs.	micrograms	lipids_biota
C15_0	Mass of C15:0 fatty acid extracted from eggs.	micrograms	lipids_biota
C15_1	Mass of C15:1 fatty acid extracted from eggs.	micrograms	lipids_biota
C16_0	Mass of C16:0 fatty acid extracted from eggs.	micrograms	lipids_biota
C16_1	Mass of C16:1 fatty acid extracted from eggs.	micrograms	lipids_biota
C17_0	Mass of C17:0 fatty acid extracted from eggs.	micrograms	lipids_biota
C17_1	Mass of C17:1 fatty acid extracted from eggs.	micrograms	lipids_biota
C18_0	Mass of C18:0 fatty acid extracted from eggs.	micrograms	lipids_biota
C18_1n9	Mass of C18:1n9 fatty acid extracted from eggs.	micrograms	lipids_biota
cLA	Mass of cis-Linoleic Acid extracted from eggs.	micrograms	lipids_biota
tLA	Mass of trans-Linoleic Acid extracted from eggs.	micrograms	lipids_biota
GLA	Mass of Gamma-Linolenic Acid extracted from eggs.	micrograms	lipids_biota
ALA	Mass of Alpha Linoleic Acid extracted from eggs.	micrograms	lipids_biota
C20_0	Mass of C20:0 fatty acid extracted from eggs.	micrograms	lipids_biota
C20_1n9	Mass of C20:1n9 fatty acid extracted from eggs.	micrograms	lipids_biota
EicoA	Mass of Eicosadienoic Acid extracted from eggs.	micrograms	lipids_biota
DGLA	Mass of Dihomo-Gamma-Linolenic Acid extracted from eggs.	micrograms	lipids_biota
AA	Mass of Arachidonic Acid extracted from eggs.	micrograms	lipids_biota
ETE	Mass of Eicosatrienoic Acid extracted from eggs.	micrograms	lipids_biota
EPA	Mass of Eicosapentaenoic Acid extracted from eggs.	micrograms	lipids_biota
DHA	Mass of Docosahexaenoic Acid extracted from eggs.	micrograms	lipids_biota
O3	Mass of Omega-3 Fatty Acids extracted from eggs.	micrograms	lipids_biota
O6	Mass of Omega-6 Fatty Acids extracted from eggs.	micrograms	lipids_biota
O3_O6	Ratio of the masses of the Omega-3 and Omega-6 Fatty Acids extracted from eggs	unitless	lipids_biota
EPA_DHA	Ratio of the masses of Eicosapentaenoic Acid and Docosahexaenoic Acid extracted from eggs	unitless	lipids_biota
HUFA	Mass of the highly-unsaturated Fatty Acids extracted from eggs.	micrograms	lipids_biota
OFA	Mass of the odd-numbered Fatty Acids extracted from eggs.	micrograms	lipids_biota
C14_0_Egg	Concentration of C14:0 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
C14_1_Egg	Concentration of C14:1 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
C15_0_Egg	Concentration of C15:0 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
C15_1_Egg	Concentration of C15:1 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
C16_0_Egg	Concentration of C16:0 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
C16_1_Egg	Concentration of C16:1 fatty as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
C17_0_Egg	Concentration of C17:0 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
C17_1_Egg	Concentration of C17:1 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
C18_0_Egg	Concentration of C18:0 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
C18_1n9_Egg	Concentration of C18:1n9 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted..	unitless	lipids_biota
cLA_Egg	Concentration of cis-Linoleic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
tLA_Egg	Concentration of trans-Linoleic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
GLA_Egg	Concentration of Gamma-Linolenic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
ALA_Egg	Concentration of Alpha Linoleic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
C20_0_Egg	Concentration of C20:0 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
C20_1n9_Egg	Concentration of C20:1n9 fatty acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
EicoA_Egg	Concentration of Eicosadienoic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
DGLA_Egg	Concentration of Dihomo-Gamma-Linolenic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
AA_Egg	Concentration of Arachidonic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
ETE_Egg	Concentration of Eicosatrienoic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
EPA_Egg	Concentration of Eicosapentaenoic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
DHA_Egg	Concentration of Docosahexaenoic Acid as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
O3_Egg	Concentration of Omega-3 Fatty Acids as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
O6_Egg	Concentration of Omega-6 Fatty Acids as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
HUFA_Egg	Concentration of the highly-unsaturated Fatty Acids as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota
OFA_Egg	Concentration of the odd-numbered Fatty Acids as the proportion of the mass of the eggs from which the fatty acids were extracted.	unitless	lipids_biota