Dataset: Metabolomic fingerprints of individual algal cells using the Single-Probe Mass Spectrometry technique from experiments conducted between May and August of 2017

This dataset has not been validatedPreliminary and in progressVersion 1 (2018-09-21)Dataset Type:experimental

Principal Investigator: Boris Wawrik (University of Oklahoma)

Co-Principal Investigator: Zhibo Yang (University of Oklahoma)

BCO-DMO Data Manager: Amber D. York (Woods Hole Oceanographic Institution)

Project: Collaborative Research: Creatine Cycling in Marine Bacterial and Phytoplankton Assemblages (Creatine Cycling)

Abstract

Single-probe Mass Spectrometry was used to obtain mass to charge ratio (m/z) and relative intensity values of marine dinoflagellate Scrippsiella trochoidea cells grown under different illumination levels and under nitrogen (N) limiting conditions.

Cite Dataset

These data were published in Sun et al., 2018.

A tabular version of this dataset is accessible by clicking the "Get Data" button on this page.  The columns of the tabular dataset are described in the "Parameters" section on this page.  This dataset is available as standard MS file format (.mzML) files within the following tar.gz file:
Algal_Cell_Data_mzML.tar.gz (2.4 GB, contains 20 .mzML files)

.mzML is a standard MS file format that can be viewed using freeware such as mMass (http://www.mmass.org/) and ProteoWizard (http://proteowizard.sourceforge.net/index.shtml).

Related Datasets

Different Version
Dataset: https://doi.org/10.5281/zenodo.1188486
Mei, S., Yang, Z., & Wawrik, B. (2018). Mass Spectra For Single Scrippsiella Trochoidea Cells: Light Vs. Dark & Replete Vs. N Limiting Conditions (Version 1) [Data set]. Zenodo. https://doi.org/10.5281/zenodo.1188486

Related Publications

Results
Sun, M., Yang, Z., & Wawrik, B. (2018). Metabolomic Fingerprints of Individual Algal Cells Using the Single-Probe Mass Spectrometry Technique. Frontiers in Plant Science, 9. doi:10.3389/fpls.2018.00571
Methods
Guijas, C., Montenegro-Burke, J. R., Domingo-Almenara, X., Palermo, A., Warth, B., Hermann, G., … Siuzdak, G. (2018). METLIN: A Technology Platform for Identifying Knowns and Unknowns. Analytical Chemistry, 90(5), 3156–3164. doi:10.1021/acs.analchem.7b04424
Methods
Guillard, R. R. L., & Hargraves, P. E. (1993). Stichochrysis immobilis is a diatom, not a chrysophyte. Phycologia, 32(3), 234–236. doi:10.2216/i0031-8884-32-3-234.1
Methods
Guillard, R. R. L., & Ryther, J. H. (1962). STUDIES OF MARINE PLANKTONIC DIATOMS: I. CYCLOTELLA NANA HUSTEDT, AND DETONULA CONFERVACEA (CLEVE) GRAN. Canadian Journal of Microbiology, 8(2), 229–239. doi:10.1139/m62-029
Methods
Guillard, R. R. L., Kilham, P., & Jackson, T. A. (1973). KINETICS OF SILICON-LIMITED GROWTH IN THE MARINE DIATOM THALASSIOSIRA PSEUDONANA HASLE AND HEIMDAL (= CYCLOTELLA NANA HUSTEDT)2. Journal of Phycology, 9(3), 233–237. doi:10.1111/j.1529-8817.1973.tb04086.x

Wawrik, B., Yang, Z. (2018) Metabolomic fingerprints of individual algal cells using the Single-Probe Mass Spectrometry technique from experiments conducted between May and August of 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-09-21 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/746185 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

NSF Logo©2020 Biological and Chemical Oceanography Data Management Office.
Funded by the U.S. National Science Foundation