Contributors | Affiliation | Role |
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Zimmerman, Richard C. | Old Dominion University (ODU) | Principal Investigator |
Burdige, David J. | Old Dominion University (ODU) | Co-Principal Investigator |
Copley, Nancy | Woods Hole Oceanographic Institution (WHOI BCO-DMO) | BCO-DMO Data Manager |
This dataset includes results of carbon and nitrogen analyses on seagrasses collected in the Virginia Coastal Lagoons and from St. George Sound FL.
Seagrass samples were collected randomly from each site by hand using 6" diameter PVC cores pounded into the sediment. Intact plants, including roots and rhizomes, were separated from the sediment and organic debris. The green shoots were separated from roots & rhizomes, rinsed in fresh water and dried separately at -60° C. Dried samples were ground in a mortar and pestle using liquid nitrogen and re-dried. The dry powder was analyzed for percent Carbon, percent Nitrogen, delta-13C, and delta-15N by the Stable Isotope Facility, U.C. Davis.
Samples were collected by hand, using hand tools. Chemical and isotope analyses were performed by the Stable Isotope Facility, UC Davis using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK).
Data were segregated by location, tissue type, and species. Means and errors were calculated from 10 to 90 replicate samples (separate plants).
BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- added columns for site, lat, and lon
File |
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seagrass_CN.csv (Comma Separated Values (.csv), 61.58 KB) MD5:a3639cf38133405082c71df33f74f582 Primary data file for dataset ID 746307 |
Parameter | Description | Units |
Location | Location of sample collection | unitless |
lat | latitude; north is positive | decimal degrees |
lon | longitude; east is postive | decimal degrees |
Sample_ID | sample identifier | unitless |
d13C | the ratio of stable isotopes 13C:12C | unitless |
C_Amount_ug | mass of Carbon in the sample | micrograms |
d13C_Comment | comments pertaining to delta 13C analysis | unitless |
d15N | the ratio of stable isotopes 15N:14N | unitless |
N_Amount_ug | mass of Nitrogen in the sample | micrograms |
Dry_Mass_mg | dry mass of sample | milligrams |
pcnt_C_g_per_gDW | percent Carbon in sample dry weight | unitless (grams/grams) |
pcnt_N_g_per_gDW | percent Nitrogen in sample dry weight | unitless (grams/grams) |
C_to_N | Carbon to Nitrogen ratio | unitless (mol/mol) |
d15N_Comment | comments pertaining to delta 15N analysis | unitless |
Tray_Name | sample tray identifier | unitless |
Well_Id | well identifier | unitless |
Type_of_Material | type of plant material; leaf or root/rhyzome | unitless |
Analysis | type of analyses performed on sample | unitless |
OurLabID | intermal sample identifier assigned by researcher's laborator | unitless |
Analysis_Number | sample identifier assigned by Stable Isotope Facility | unitless |
Dataset-specific Instrument Name | PDZ Europa ANCA-GSL elemental analyzer |
Generic Instrument Name | Elemental Analyzer |
Dataset-specific Description | Used for chemical and isotope analyses. |
Generic Instrument Description | Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. |
Dataset-specific Instrument Name | PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK) |
Generic Instrument Name | Isotope-ratio Mass Spectrometer |
Dataset-specific Description | Used for chemical and isotope analyses. |
Generic Instrument Description | The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). |
NSF abstract:
This research will develop a quantitative understanding of the factors controlling carbon cycling in seagrass meadows that will improve our ability to quantify their potential as blue carbon sinks and predict their future response to climate change, including sea level rise, ocean warming and ocean acidification. This project will advance a new generation of bio-optical-geochemical models and tools (ECHOES) that have the potential to be transform our ability to measure and predict carbon dynamics in shallow water systems.
This study will utilize cutting-edge methods for evaluating oxygen and carbon exchange (Eulerian and eddy covariance techniques) combined with biomass, sedimentary, and water column measurements to develop and test numerical models that can be scaled up to quantify the dynamics of carbon cycling and sequestration in seagrass meadows in temperate and tropical environments of the West Atlantic continental margin that encompass both siliciclastic and carbonate sediments. The comparative analysis across latitudinal and geochemical gradients will address the relative contributions of different species and geochemical processes to better constrain the role of seagrass carbon sequestration to global biogeochemical cycles. Specifically the research will quantify: (i) the relationship between C stocks and standing biomass for different species with different life histories and structural complexity, (ii) the influence of above- and below-ground metabolism on carbon exchange, and (iii) the influence of sediment type (siliciclastic vs. carbonate) on Blue Carbon storage. Seagrass biomass, growth rates, carbon content and isotope composition (above- and below-ground), organic carbon deposition and export will be measured. Sedimentation rates and isotopic composition of PIC, POC, and iron sulfide precipitates, as well as porewater concentrations of dissolved sulfide, CO2, alkalinity and salinity will be determined in order to develop a bio-optical-geochemical model that will predict the impact of seagrass metabolism on sediment geochemical processes that control carbon cycling in shallow waters. Model predictions will be validated against direct measurements of DIC and O2 exchange in seagrass meadows, enabling us to scale-up the density-dependent processes to predict the impacts of seagrass distribution and density on carbon cycling and sequestration across the submarine landscape.
Status, as of 09 June 2016: This project has been recommended for funding by NSF's Division of Ocean Sciences.
Funding Source | Award |
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NSF Division of Ocean Sciences (NSF OCE) | |
NSF Division of Ocean Sciences (NSF OCE) |