Dataset: Series 3B: A-pulse raw fluorescence

Name: Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) - growth under bubbling stress: A-pulse raw fluorescence data
Published: 2018-10-31
Keywords: biota, oceans

Cite This Dataset

Temporal Extent: 2018-07-03 - 2018-07-06

Project:

Collaborative Research: Effects of multiple stressors on Marine Phytoplankton (Stressors on Marine Phytoplankton)

Principal Investigator:

Uta Passow (University of California-Santa Barbara, UCSB-MSI)

Co-Principal Investigator:

Edward Laws (Louisiana State University College of the Coast and Environment, LSU-CC&E [formerly SC&E])

Scientist:

Nigel D'Souza (University of California-Santa Barbara, UCSB-MSI)

Contact:

Nigel D'Souza (University of California-Santa Barbara, UCSB-MSI)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2018-10-31

Restricted:

Release Date:

2019-07-01

Validated:

Yes

Current State:

Final no updates expected

Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) - growth under bubbling stress: A-pulse raw fluorescence data

Abstract:

Experiments were conducted to determine the optimal actinic light pulse (A-pulse) setting for NPQ1 protocols of the AquaPen for assessment of non-photochemical quenching in Thalassiosira pseudonana CCMP 1014 cultures grown at different light environments. These are the raw fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C at four light levels and pulsed at eight light levels.

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Description

Methods & Sampling

Experiments were conducted in the lab at the University of California Santa Barbara to determine the optimal actinic light pulse (A-pulse) setting for NPQ1 protocols of the AquaPen for assessment of non-photochemical quenching in Thalassiosira pseudonana CCMP 1014 cultures grown at different light environments. TP1014 stock cultures were maintained in artificial sea water (Kester et. al 1967), enriched as with f/2 media (Guillard 1975). For the experiment, 5 ml of the TP1014 stock cultures was inoculated into 75 ml of ASW in four tubes. The tubes were incubated in a Multicultivator MC-1000 OD unit (Qubit Systems), at 25°C and light intensities of 200, 400, 600 and 800 µmol photons·m-2·sec-1 respectively, set at a 12:12 day:night cycle for four days. All samples were bubbled with air at 60 ml·min-1 through a 0.2 µm stainless steel carbonating stones. Samples were collected from each tube after three days and analyzed used for assessment of photochemistry using the Aquapen-C AP-C 100 (Photon Systems Instruments). Three ml samples were placed in the dark at 25°C for a minimum of 30 minutes prior to measuring photochemistry. The NPQ1 protocol on the instrument was used for assessment of non-photochemical quenching in samples. The NPQ1 protocol administers 5 light pulses over 60 seconds during actinic light exposure, followed by 3 light pulses over 88 seconds during recovery in the dark. Blue light (455 nm) was used as actinic light in these experiments. Baseline measurements were made at f-pulse settings of 0.03 μmol · m-2 · s-1, Saturating pulses were set at 2100 μmol · m-2 · s-1, and actinic light pulses (for the NPQ1 protocol only) were set at 100, 200, 300, 400, 500, 600, 700, and 800 µmol photons·m-2·sec-1 respectively for each sample.

Data Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions

Data Files

File
3B_Apulse_test_raw.csv (Comma Separated Values (.csv), 25.91 KB) MD5:b6613bd4186672f7c51c51fc18e44f11 Primary data file for dataset ID 748930

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1538602

Deployments

None available yet

Instruments

Cell Cultivator

Supplied Name: Multicultivator MC-1000 OD (Qubit Systems)

Supplied Description:

Used for incubation of TP1014 cultures.

Instrument Type

Generic Name: Cell Cultivator

Generic Description:

An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.

Fluorometer

Supplied Name: Aquapen-C AP-C 100 (Photon Systems Instruments)

Supplied Description:

Used to measure fluorescence.

A hand-held cuvette version of the FluorPen fluorometer equipped with a blue and red LED emitter. Blue excitation light (455 nm) is intended for chlorophyll excitation, i.e., for measuring chlorophyll fluorescence in algal cultures. Red-orange excitation light (620 nm) is intended for excitation through phycobilins and is suitable for measuring in cyanobacteria.

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Time	time elapsed in between fluorescence measurements	microseconds	time
flr_T200_AP100	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 100 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T200_AP200	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 200 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T200_AP300	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 300 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T200_AP400	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 400 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T200_AP500	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 500 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T200_AP600	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 600 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T200_AP700	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 700 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T200_AP800	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 800 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T400_AP100	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 100 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T400_AP200	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 200 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T400_AP300	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 300 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T400_AP400	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 400 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T400_AP500	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 500 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T400_AP600	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 600 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T400_AP700	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 700 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T400_AP800	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 800 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T600_AP100	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 100 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T600_AP200	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 200 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T600_AP300	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 300 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T600_AP400	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 400 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T600_AP500	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 500 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T600_AP600	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 600 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T600_AP700	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 700 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T600_AP800	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 800 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T800_AP100	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 100 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T800_AP200	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 200 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T800_AP300	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 300 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T800_AP400	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 400 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T800_AP500	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 500 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T800_AP600	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 600 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T800_AP700	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 700 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor
flr_T800_AP800	fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 800 micromol photons/meter^2/second	Relative fluorescence units (RFU)	fluor

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Dataset: Series 3B: A-pulse raw fluorescence