Dataset: Series 3B: Supplemental experiments on Thalassiosira pseudonana (CCMP1014) cultures determining the optimal aquapen actinic light pulse (A-pulse) setting: computed values including min and max fluorescence

ValidatedRelease Date:2019-07-01Final no updates expectedVersion 1 (2018-10-29)Dataset Type:experimental

Principal Investigator: Uta Passow (University of California-Santa Barbara)

Co-Principal Investigator: Edward Laws (Louisiana State University College of the Coast and Environment)

Scientist, Contact: Nigel D'Souza (University of California-Santa Barbara)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Effects of multiple stressors on Marine Phytoplankton (Stressors on Marine Phytoplankton)


Abstract

Series 3B: Supplemental experiments on Thalassiosira pseudonana (CCMP1014) cultures determining the optimal aquapen actinic light pulse (A-pulse) setting for NPQ1 protocols of the AquaPen for assessment of non-photochemical quenching in Thalassiosira pseudonana CCMP 1014 cultures grown at different light environments. Computed values include min and max fluorescence in dark-adapted state, quantum yield of the Photosystem II, non-photochemical chlorophyll fluorescence quenching and the coefficien...

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Experiments were conducted to determine the optimal actinic light pulse (A-pulse) setting for NPQ1 protocols of the AquaPen for assessment of non-photochemical quenching in Thalassiosira pseudonana CCMP 1014 cultures grown at different light environments.

Computed values include min and max fluorescence in dark-adapted state, quantum yield of the Photosystem II, non-photochemical chlorophyll fluorescence quenching and the coefficient of photochemical quenching (an estimate of open PSII reaction centers during these corresponding measurements).


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Methods

Guillard, R. R. L. (1975). Culture of Phytoplankton for Feeding Marine Invertebrates. Culture of Marine Invertebrate Animals, 29–60. doi:10.1007/978-1-4615-8714-9_3
Methods

Kester, D. R., Duedall, I. W., Connors, D. N., & Pytkowicz, R. M. (1967). Preparation of Artificial Seawater 1. Limnology and Oceanography, 12(1), 176–179. doi:10.4319/lo.1967.12.1.0176