Table of Contents

• Dataset Description
  • Data Processing Description
• Data Files
• Related Publications
• Parameters
• Project Information
• Funding

Mussel larvae of Mytilus galloprovincialis were grown in two pH treatments (pH 8.1 and 7.4). Larvae were collected for biological measurements of shell field development and calcification at 35 hours post-fertilization (hpf, trochophore stage). Calcein dye was added to the cultures prior to the start of calcification. Calcofluor is live dye and so was added to sampled larvae at 35 hpf for immediate imaging. Confocal microscopy was used for 3D imaging of larvae. Images were processed in ImageJ. Shell field area was determined as the area stained by calcofluor, on one valve. Calcification area was determined as the area stained by calcein, on one valve. See publication for details. 

BCO-DMO Processing Notes:

• added conventional header with dataset name, PI name, version date

NSF abstract:

One of the major goals of ocean acidification research is to understand how ecosystem functioning and services will change in the future. In this project, the fellow will assess the influence of pH variability on an ecologically and economically important mussel species, under future ocean pH and temperature conditions. The research will be conducted at Laboratoire d'Océanographie de Villefranche-sur-Mer, France in collaboration with international host scientist Dr. Jean-Pierre Gattuso and sponsoring scientist Dr. Todd Martz (Scripps Institution of Oceanography, USA). By hosting a workshop, the fellow will introduce pH sensors to European students and scientists and promote the use of field data in the design of biological experiments. The project supports the training of a postdoctoral fellow and two undergraduate student interns. Results and data from this project will be disseminated at conferences and through open-access publications and data repositories.

Experiments investigating the effects of ocean acidification on marine organisms often ignore the spatio-temporal variability in seawater pH that is present in coastal marine ecosystems. Such heterogeneity in pH may provide temporal refuge from corrosive seawater under future levels of acidification. Utilizing a combination of field and lab experiments, this project will evaluate the influence of pH variability and interactive effects of warming and acidification on mussel physiology through several levels of biological organization. Should variability in pH provide beneficial effects on mussel development and growth, results of the project provide an avenue for local management of ocean acidification in coastal regions and aquaculture practices.

This project produced the following publications:

Kapsenberg, L., Miglioli, A., Bitter, M. C., Tambutté, E., Dumollard, R., and Gattuso, J. P. (2018) Ocean pH fluctuations affect mussel larvae at key developmental transitions, Proceedings of the Royal Society B: Biological Sciences, 285, 20182381, doi: 10.1098/rspb.2018.2381.

Kapsenberg, L, EE Bockmon, PJ Bresnahan, KJ Kroeker, J-P Gattuso, and TR Martz (2017) Advancing ocean acidification biology using Durafet® pH electrodes. Frontiers in Marine Science 4: 321. doi:10.3389/fmars.2017.00321

Kapsenberg, L, S Alliouane, F Gazeau, L Mousseau, and JP Gattuso (2017) Coastal ocean acidification and increasing total alkalinity in the northwestern Mediterranean Sea. Ocean Science 13: 411-426. doi:10.5194/os-13-411-2017

Kapsenberg, L, DK Okamoto, J Dutton, and GE Hofmann (2017) Sensitivity of sea urchin fertilization to pH varies across a natural pH mosaic. Ecology and Evolution 7: 1737-1750. doi:10.1002/ece3.2776