Dataset: 16S rRNA gene amplicon sequences from FLOCS experiments at the Juan de Fuca Ridge CORKs during August 2014.

ValidatedFinal no updates expectedVersion 1 (2019-02-20)Dataset Type:Cruise Results

Principal Investigator: Beth N. Orcutt (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator: Gustavo Ramirez (University of Southern California)

BCO-DMO Data Manager: Mathew Biddle (Woods Hole Oceanographic Institution)


Program: Center for Dark Energy Biosphere Investigations (C-DEBI)

Project: Collaborative Research: Completing single- and cross-hole hydrgeologic and microbial experiments: Juan de Fuca Flank (JdF Flank)

The DNA data from the Juan de Fuca project are publicly available through NCBI Short Read Archive under BioProject PRJNA472057; BioSample accession numbers: SAMN09228041- SAMN09228055 

species: uncultivated marine metagenome

description of the types of sequences: Illumina amplicon sequences of the V1-V3 hypervariable region of the 16S rRNA gene

locations where species were collected: IODP holes U1301A, U1362A, and U1362B on the Juan de Fuca Ridge flank, 47.45N/127.45W


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Related Publications

Methods

Fisher, A. T., Wheat, C. G., Becker, K., Cowen, J., Orcutt, B., Hulme, S., … Edwards, K. J. (2011). Design, deployment, and status of borehole observatory systems used for single-hole and cross-hole experiments, IODP Expedition 327, eastern flank of Juan de Fuca Ridge. Proceedings of the IODP. doi:10.2204/iodp.proc.327.107.2011
Methods

Orcutt, B. N., Bach, W., Becker, K., Fisher, A. T., Hentscher, M., Toner, B. M., … Edwards, K. J. (2010). Colonization of subsurface microbial observatories deployed in young ocean crust. The ISME Journal, 5(4), 692–703. doi:10.1038/ismej.2010.157