Dataset: Picocyanobacteria TEP: Cell Abundance

Name: Cell abundance of xenic and axenic marine Prochlorococcus and Synechococcus cultures from laboratory experiments conducted in 2016 and 2017
Published: 2019-08-13
Keywords: oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.774628.1

Temporal Extent: 2016 - 2017

Project:

Aggregation of Marine Picoplankton (Marine Plankton Aggregation)

Principal Investigator:

Susanne Neuer (Arizona State University, ASU)

Student:

Bianca N. Cruz (Arizona State University, ASU)

Contact:

Susanne Neuer (Arizona State University, ASU)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2019-08-13

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Cell abundance of xenic and axenic marine Prochlorococcus and Synechococcus cultures from laboratory experiments conducted in 2016 and 2017

Abstract:

Cell abundance of xenic and axenic marine Prochlorococcus and Synechococcus cultures from laboratory experiments conducted in 2016 and 2017.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2019-08-13	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.774628.1	2019-12-24

Description

Cell abundance of xenic and axenic marine Prochlorococcus and Synechococcus cultures from laboratory experiments conducted in 2016 and 2017.

These data were published in Cruz and Neuer, 2019.

Related datasets from the same laboratory experiments:
Picocyanobacteria TEP: Growth Rates https://www.bco-dmo.org/dataset/774634
Picocyanobacteria TEP: Suspended Aggregates https://www.bco-dmo.org/dataset/774639
Picocyanobacteria TEP: TEP Concentration https://www.bco-dmo.org/dataset/774646

Methods & Sampling

Methodology:

Duplicate cultures of Synechococcus WH8102 (axenic), Synechococcus WH7805 (xenic) and Prochlorococcus marinus MED4 (xenic and axenic) were sampled every other day for 17-19 days for the quantification of single cells, suspended aggregates (aggregates ca. 5-60 μm), and TEP. Single cell abundance was determined using epifluorescence microscopy.

Data Processing Description

BCO-DMO Data Manager Processing Notes: Growth Rates
* Excel sheet extracted to a csv file
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions
* blank values in this dataset are displayed as "nd" for "no data." nd is the default missing data identifier in the BCO-DMO system.

Data Files

File
cell_abund.csv (Comma Separated Values (.csv), 2.81 KB) MD5:92190ed26e2e7eee691b5f4c5ab76e0b Primary data file for dataset ID 774628

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1658527

Deployments

None available yet

Instruments

Fluorescence Microscope

Supplied Name: AxioScope.A1

Supplied Description:

AxioScope.A1, Carl Zeiss, Germany

Instrument Type

Generic Name: Fluorescence Microscope

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Culture	Culture name	unitless	sample_descrip
Days	Days since experiment start	count (days)	time_elapsed
Cell_Abundance_rep1	Cell abundance as determined using epifluorescence microscopy for replicate 1.	cells per mL	abundance
Cell_Abundance_rep2	Cell abundance as determined using epifluorescence microscopy for replicate 2.	cells per mL	abundance