Dataset: NCBI accessions and metadata associated with Caribbean sponge metagenomes collected from Curacao, Belize, Cayman Islands and St. Croix, 2009 and 2017-2018

ValidatedFinal no updates expectedDOI: 10.1575/1912/bco-dmo.775451.1Version 1 (2019-08-15)Dataset Type:Other Field Results

Principal Investigator: Michael P. Lesser (University of New Hampshire)

Co-Principal Investigator: Marc Slattery (University of Mississippi)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Program: Dimensions of Biodiversity (Dimensions of Biodiversity)

Project: Collaborative Research: Dimensions: Evolutionary Ecology of Sponges and Their Microbiome Drives Sponge Diversity on Coral Reefs (DimensionsSponge)


Abstract

NCBI accessions and metadata associated with Caribbean sponge metagenomes collected from Curacao, Belize, Cayman Islands and St. Croix, 2009 and 2017-2018.

This dataset includes NCBI Accessions for Raw sequence data for 16S rRNA amplicon microbiomes.

The NCBI BioProject number is PRJNA555077 which covers 1547 samples of 16S libraries where the metadata are also available but the sequences themselves are still embargoed. [2019-08-15]

Samples were collected at 15 m by SCUBA diving in the Caribbean near Curacao, Belize, Cayman Islands and St. Croix, in 2009 and 2017-2018. Samples were cleaned, frozen whole, or placed in a DNA/RNA preservation buffer and shipped back to the University of New Hampshire where DNA was extracted and metagenetic, shotgun or cDNA sequencing was conducted.

Data was quality controlled and analyzed in spreadsheets for descriptive statistics, and statistical software was used for bioinformatic analyses.


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