Dataset: Calibration of the Isotope Ratio Mass Spectrometer vs. aliquots of 15N2 gas diluted in air then helium

ValidatedFinal no updates expectedDOI: 10.1575/1912/bco-dmo.778000.1Version 1 (2019-10-02)Dataset Type:experimental

Principal Investigator: Julie Granger (University of Connecticut)

Co-Principal Investigator: Annie Bourbonnais (University of Massachusetts Dartmouth)

Co-Principal Investigator: Samuel Wilson (University of Hawai'i)

BCO-DMO Data Manager: Mathew Biddle (Woods Hole Oceanographic Institution)


Project: EAGER: Collaborative Research: Detection limit in marine nitrogen fixation measurements - Constraints of rates from the mesopelagic ocean (EAGER NitFix)


Abstract

In order to verify that measurements of the 15N2 atom% of dissolved N2 gas samples effectuated by Membrane Inlet Mass Spectrometry are accurate, we compared these to corresponding measurements of parallel samples on a Delta V Isotope Ratio Mass Spectrometer (IRMS). To this end, we first ensured that the IRMS returns accurate 15N2 atom% values, by measuring the 15N2 atom% of a serial dilutions of a 15N2 gas stock in air and helium. This calibration is documented herein, showing that IRMS measurem...

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Measurements of the 15N2 atom % in prepared standards and solutions.


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Results

White, A. E., Granger, J., Selden, C., Gradoville, M. R., Potts, L., Bourbonnais, A., Fulweiler, R. W., Knapp, A. N., Mohr, W., Moisander, P. H., Tobias, C. R., Caffin, M., Wilson, S. T., Benavides, M., Bonnet, S., Mulholland, M. R., & Chang, B. X. (2020). A critical review of the 15N2 tracer method to measure diazotrophic production in pelagic ecosystems. Limnology and Oceanography: Methods, 18(4), 129–147. Wiley. https://doi.org/10.1002/lom3.10353