Dataset: C:N in evolved and control populations

Name: C:N ratios of two heat‐tolerant populations of Chaetoceros simplex and control and ancestral populations, at different temperatures
Published: 2019-10-07
Keywords: biota, oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.778926.1

Spatial Extent: N:42.4061 E:-85.4007 S:42.4061 W:-85.4007

Temporal Extent: 2016-06 - 2016-10

Project:

Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients (Phytoplankton Community Responses)

Program:

Dimensions of Biodiversity (Dimensions of Biodiversity)

Principal Investigator:

Elena Litchman (Michigan State University, MSU)

Co-Principal Investigator:

Christopher Klausmeier (Michigan State University, MSU)

Scientist:

Colin T. Kremer (Michigan State University, MSU)

Contact:

Maria Aranguren-Gassis (Universidad de Vigo)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2019-10-07

Restricted:

Validated:

Yes

Current State:

Final no updates expected

C:N ratios of two heat‐tolerant populations of Chaetoceros simplex and control and ancestral populations, at different temperatures

Abstract:

C:N ratios of the two 34C‐tolerant populations of Chaetoceros simplex and control and ancestral populations, at different temperatures.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2019-10-07	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.778926.1	2019-10-28

Description

C:N ratios of the two 34C‐tolerant populations of Chaetoceros simplex and control and ancestral populations, at different temperatures.

Methods & Sampling

Chaetoceros simplex cultures, were obtained from population strain CCMP 200 (National Center for Marine Algae and Microbiota, NCMA).

C : N elemental analyses:
We measured the elemental composition of the two 34 °C‐tolerant populations and the control and ancestral populations, at different temperatures. From each culture during exponential growth, we filtered 10–20 mL duplicate subsamples onto pre‐combusted GF/F filters. The filters were then dried at 60 °C for 24 h, packed in aluminium tins and kept in a desiccator. Blanks were 10–20 mL of the medium filtered and processed as other samples. Particulate C and N were measured with a CHN analyzer (Costech ECS 4010) and ratios were calculated from weight percentage of each element after the subtraction of the corresponding blank.

More details in Aranguren-Gassis et al. 2019, Ecology Letters.

Data Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions

Data Files

File
CN.csv (Comma Separated Values (.csv), 1.08 KB) MD5:138a870595447d07c7752333ec25eefd Primary data file for dataset ID 778926

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1638804
NSF Division of Ocean Sciences	OCE-1638834
NSF Division of Ocean Sciences	OCE-1638958

Deployments

None available yet

Instruments

CHN Elemental Analyzer

Supplied Name: CHN analyzer (Costech ECS 4010)

Supplied Description:

Instrument Type

Generic Name: CHN Elemental Analyzer

Acronym: CHN_EA

Generic Description:

A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Temperature	Culture maintenance temperature	Celsius degrees	temp
CN_ratio	Carbon:Nitrogen ratio	percent	C_to_N
treatment	Experimental treatment: Evolved = populations raised 34C; Control = populations maintained at 25C	unitless	treatment