Dataset: Continuous culture studies of possible climate change effects: Thalassiosira pseudonana CCMP1335 growth in nitrate-limited and nutrient-replete cultures

ValidatedRelease Date:2020-09-30Final no updates expectedDOI: 10.26008/1912/bco-dmo.779368.1Version 1 (2020-05-07)Dataset Type:experimental

Principal Investigator: Edward Laws (Louisiana State University College of the Coast and Environment)

Co-Principal Investigator: Uta Passow (University of California-Santa Barbara)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Effects of multiple stressors on Marine Phytoplankton (Stressors on Marine Phytoplankton)


Abstract

The marine diatom Thalassiosira pseudonana clone CCMP 1335 was grown in a continuous culture system on a 14:10 light-dark cycle under either nitrate-limited or nutrient-replete conditions, a photoperiod irradiance of either 50 or 300 micro-mol photons per square meter per second, partial pressures of either 400 or 1000 ppm CO2, and temperatures ranging from 5 to 32 degrees Celsius. Growth rates, photosynthetic rates, respiration rates, C:N ratios, C:Chlorophyll-a ratios, productivity indices, Fv...

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The marine diatom Thalassiosira pseudonana clone CCMP 1335 was grown in a continuous culture system on a 14:10 light-dark cycle under either nitrate-limited or nutrient-replete conditions, a photoperiod irradiance of either 50 or 300 micro-mol photons per square meter per second, partial pressures of either 400 or 1000 ppm CO2, and temperatures ranging from 5 to 32 degrees Celsius. Growth rates, photosynthetic rates, respiration rates, C:N ratios, C:Chlorophyll-a ratios, productivity indices, Fv/Fm ratios, and the initial slope and light-saturated asymptote of short-term photosynthesis-irradiance curves are reported.


Related Datasets

Replaces Old Versions

Dataset: [Deprecated] Thalassiosira pseudonanana in nitrate replete culture
Laws, E., Passow, U. (2017) [Deprecated] Laboratory growth, photosynthetic, and growth rates of Thalassiosira pseudonana clone 3H in nitrate replete culture (Stressors on Marine Phytoplankton project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2017-09-14 http://lod.bco-dmo.org/id/dataset/715193
Replaces Old Versions

Dataset: [Deprecated] Thalassiosira pseudonanana in nitrate limited culture
Laws, E., Passow, U. (2017) [Deprecated] Laboratory growth, photosynthetic, and respiration rates of Thalassiosira pseudonana clone 3H in nitrate limited culture (Stressors on Marine Phytoplankton project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2017-09-14 http://lod.bco-dmo.org/id/dataset/714846

Related Publications

Methods

Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp. ISBN: 1-897176-07-4. URL: https://www.nodc.noaa.gov/ocads/oceans/Handbook_2007.html
Methods

Sunda, W. G., & Ransom Hardison, D. (2007). Ammonium uptake and growth limitation in marine phytoplankton. Limnology and Oceanography, 52(6), 2496–2506. doi:10.4319/lo.2007.52.6.2496