Dataset: T. pseudonana fatty acids by biovolume

Name: Fatty acid profiles by biovolume of replicate populations of Thalassiosira pseudonana, selected at 16 and 31C for ~500 generations and assayed at 4 temperatures
Published: 2019-10-28
Keywords: biota, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.780155.1

Spatial Extent: N:42.4061 E:-85.4007 S:42.4061 W:-85.4007

Temporal Extent: 2017-12-09 - 2018-01-05

Project:

Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients (Phytoplankton Community Responses)

Program:

Dimensions of Biodiversity (Dimensions of Biodiversity)

Principal Investigator:

Elena Litchman (Michigan State University, MSU)

Co-Principal Investigator:

Daniel R. O'Donnell (Michigan State University, MSU)

Contact:

Daniel R. O'Donnell (Michigan State University, MSU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2019-10-28

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Fatty acid profiles by biovolume of replicate populations of Thalassiosira pseudonana, selected at 16 and 31C for ~500 generations and assayed at 4 temperatures

Abstract:

Fatty acid profiles by biovolume of replicate populations of Thalassiosira pseudonana, selected at 16 and 31C for ~500 generations and assayed at 4 temperatures.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2019-10-28	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.780155.1	2021-01-28

Description

Fatty acid profiles by biovolume of replicate populations of Thalassiosira pseudonana, selected at 16 and 31C for ~500 generations and assayed at 4 temperatures.

These data were published in O'Donnell and Litchman (2019) Evol. Appl. and can also be found in Supporting Information file eva12798-sup-0001-Supinfo.docx, Figures 1-5 of the main text, and on Dryad: https://datadryad.org/stash/dataset/doi:10.5061/dryad.pn04m84.

Methods & Sampling

Details of the long-term selection experiment can be found in another dataset associated with this project, entitled “Temperature-growth rate curves for Thalassiosira pseudonana”, and in O’Donnell et al. (2018), Global Change Biology. After ~500 generations of experimental selection (5 replicate populations at 16°C and 5 at 31°C), we acclimated each replicate population for 10 generations at 20°C, then acclimated each at 10, 16, 26 and 31°C for an additional 10 generations. We then sub-cultured each acclimated population into triplicate batch cultures, starting with 100,000 cells in 40 ml of L1 marine culture medium in a 50 ml tissue culture flask. We grew all experimental cultures (2 selection temperatures x 5 replicate populations x 4 assay temperatures x 3 assay replicates = 120 total experimental units) to late log phase, estimating per-capita growth rates by placing each tissue culture flask in a spectrophotometer daily and measuring absorbance at 436 nm. We harvested 15 ml of culture for fatty acid analysis, retaining ~25 µl for enumeration on a CASY particle counter. The remaining volume was used for analyses not addressed here.

Cells were harvested by filtration through a 25mm Whatman GF/B filter. We extracted fatty acids from T. pseudonana cells using a solution of chloroform, methanol, and formic acid (10:20:1). We analyzed fatty acid profiles by performing fatty acid methyl ester (FAME) reactions on each GF/B filter sample (Wang and Benning 2011; adapted for algae by Boyle et al. 2012) and quantifying fatty acids using gas chromatography.

Data Processing Description

All calculations were done and data processed and analyzed using R statistical programming software, version 3.3.2. Cell volumes were estimated by measuring the length and width of T. pseudonana frustules under oil immersion at 1000x magnification, then using these measurements to calculate the volume of a cylinder (π x rˆ2 x h). We thusly measured 10 cells per sample (10 x 120 = 1200 total cells). Mean per-biovolume fatty acid contents were estimated using these volumes.

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- removed duplicate mol_w3_bv column

Data Files

File
fatty_acids_biovol.csv (Comma Separated Values (.csv), 61.21 KB) MD5:7fb151634b4b20380a1a479cdf639595 Primary data file for dataset ID 780155

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1638804
NSF Division of Ocean Sciences	OCE-1638834
NSF Division of Ocean Sciences	OCE-1638958

Deployments

None available yet

Instruments

Automated Cell Counter

Supplied Name: CASY particle counter, Schärfe System GmbH, Reutlingen, Germany

Supplied Description:

Instrument Type

Generic Name: Automated Cell Counter

Acronym: ACC

Generic Description:

An instrument that determines the numbers, types or viability of cells present in a sample.

Gas Chromatograph

Supplied Name: Agilent Technologies 7890A Gas Chromatography system (Agilent Technologies)

Supplied Description:

Gas chromatograph using the Agilent capillary DB‐23 column for FAME analysis. Temperature settings: initial temperature 140°C, increased by 30°C/min to 160°C, then by 8°C/min to 240°C, and held at 240°C for 2 min.

Instrument Type

Generic Name: Gas Chromatograph

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/

Generic Description:

Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
evol_temp	Growth chamber temperature long-term selection experiment	degrees Celsius	temp
assay_temp	Growth chamber temperature during temperature-dependent growth assay	degrees Celsius	temp
evol_rep	Replicate population ID during long-term selection experiment	unitless	replicate
assay_rep	Experimental replicate during temperature-dependent fatty acid production assay (3 replicates)	unitless	replicate
numID	A sequential identifier used post-sample processing	unitless	sample
Sample	A sample ID used only to indicate a sample’s position on the GC rack. Sequential; but does not correspond to "numID"	unitless	sample
SampID	A sample ID that includes (in order) evol_temp; assay_temp; evol_rep and assay_rep	unitless	sample
strain	A second replicate population ID; also indicating selection temperature; identifies only eval_temp and evol_rep	unitless	taxon
good	Quality flag: indicates whether a sample was flagged as unusable: yes = usable & used; no = unusable & not used	unitless	q_flag
cells_ml	T pseudonana cells per ml of culture	cells/milliliter	cell_concentration
mol_total_bv	Total mols of fatty acids per cubic micron of biovolume	mol/micron cubed	lipids_biota
mol_SFA_bv	Mols of saturated fatty acid per cubic micron of biovolume	mol/micron cubed	lipids_biota
mol_UFA_bv	Mols of unsaturated fatty acid per cubic micron of biovolume	mol/micron cubed	lipids_biota
mol_MUFA_bv	Mols of monounsaturated fatty acid per cubic micron of biovolume	mol/micron cubed	lipids_biota
mol_PUFA_bv	Mols of polyunsaturated fatty acid per cubic micron of biovolume	mol/micron cubed	lipids_biota
mol_w3_bv	Mols of omega-3 fatty acid per cubic micron of biovolume	mol/micron cubed	lipids_biota
mol_bv_14_0	mol per cubic micron of 14:0 fatty acid. 14 indicates the length of the fatty acid chain (carbon atoms); 0 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_16_0	mol per cubic micron of 16:0 fatty acid. 14 indicates the length of the fatty acid chain (carbon atoms); 0 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_16_1a	mol per cubic micron of 16:0 fatty acid. 14 indicates the length of the fatty acid chain (carbon atoms); 1 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_16_1b	mol per cubic micron of 16:0 fatty acid. 14 indicates the length of the fatty acid chain (carbon atoms); 1 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_16_2a	mol per cubic micron of 16:0 fatty acid. 14 indicates the length of the fatty acid chain (carbon atoms); 2 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_16_2b	mol per cubic micron of 16:0 fatty acid. 14 indicates the length of the fatty acid chain (carbon atoms); 2 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_16_3	mol per cubic micron of 16:0 fatty acid. 14 indicates the length of the fatty acid chain (carbon atoms); 3 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_16_4	mol per cubic micron of 16:0 fatty acid. 14 indicates the length of the fatty acid chain (carbon atoms); 4 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_18_0	mol per cubic micron of 18:0 fatty acid. 18 indicates the length of the fatty acid chain (carbon atoms); 0 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_18_1_9	mol per cubic micron of 18:0 fatty acid. 18 indicates the length of the fatty acid chain (carbon atoms); 1 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_18_1_11	mol per cubic micron of 18:0 fatty acid. 18 indicates the length of the fatty acid chain (carbon atoms); 1 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_18_2	mol per cubic micron of 18:0 fatty acid. 18 indicates the length of the fatty acid chain (carbon atoms); 2 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_18_3_w3	mol per cubic micron of 18:0 fatty acid. 18 indicates the length of the fatty acid chain (carbon atoms); 3 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_20_0	mol per cubic micron of 20:0 fatty acid. 20 indicates the length of the fatty acid chain (carbon atoms); 0 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_20_5	mol per cubic micron of 20:0 fatty acid. 20 indicates the length of the fatty acid chain (carbon atoms); 5 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_bv_22_6	mol per cubic micron of 20:0 fatty acid. 20 indicates the length of the fatty acid chain (carbon atoms); 6 indicates the number of double bonds.	mol fatty acid per cubic micron	lipids_biota
mol_total	Total mols of fatty acids in a sample	mol fatty acid	lipids_biota
mcl	Mean fatty acid chain length	C atoms per fatty acid molecule.	lipids_biota
WUnSat	Mean degree of fatty acid unsaturation	Double bonds per fatty acid molecule.	lipids_biota

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Dataset: T. pseudonana fatty acids by biovolume