Dataset: Anderson & Rynearson 2020: Skeletonema CHN

Name: Elemental carbon and nitrogen data for Skeletonema species as analyzed in Anderson and Rynearson, 2020
Published: 2019-10-30
Keywords: thermal traits, species succession, niche differentiation, elemental composition, thermal tolerance, biota, oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.780386.1

Spatial Extent: N:41.566 E:-73.064 S:41.566 W:-73.064

Temporal Extent: 2015-03-03 - 2016-06-13

Project:

Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients (Phytoplankton Community Responses)

Program:

Dimensions of Biodiversity (Dimensions of Biodiversity)

Principal Investigator:

Tatiana A. Rynearson (University of Rhode Island, URI-GSO)

Contact:

Stephanie I. Anderson (University of Rhode Island, URI-GSO)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2019-10-30

Restricted:

Release Date:

2020-02-03

Validated:

Yes

Current State:

Final no updates expected

Elemental carbon and nitrogen data for Skeletonema species as analyzed in Anderson and Rynearson, 2020

Abstract:

Elemental concentrations for Skeletonema species (n=2), as analyzed in Anderson and Rynearson, 2020. This dataset includes carbon, nitrogen, and volume measurements for S. marinoi (n=3) and S. pseudocostatum (n=4) evaluated at the thermal minimum, maximum, and optimum for each strain. Strains were isolated from Narragansett Bay, Rhode Island.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2019-10-30	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.780386.1	2020-02-03

Description

This dataset includes carbon and nitrogen concentrations of Skeletonema cultures maintained at six temperatures. Strains were collected at Narragansett Bay, Rhode Island.

Methods & Sampling

Complete methods are outlined in Anderson and Rynearson, 2020, in press.

Elemental Analyses: From each culture and during exponential growth phase, >1 x 107 cells were filtered in triplicate onto precombusted 25-mm GF/F filters, and rinsed with 10 ml F/2 media. Blanks were made to correct for any elemental contribution from filters or media. Filters were dried at 60 degrees C for 24 hrs. The mass of the filter, total volume filtered, and cell counts were utilized to determine the number of cells analyzed. Elemental composition was assessed on an elemental analyzer.

Cell volume: Linear measurements of height and diameter were recorded for 30 live cells from each strain and used to calculate cell volume using the volume of a cylinder (Montagnes and Franklin 2001).

All data processing was carried out in R 3.4.1(R-Core-Team 2015).

Data Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- re-formatted date from m/d/yyyy to yyyy-mm-dd

Data Files

File
CHN.csv (Comma Separated Values (.csv), 2.86 KB) MD5:5a19430eb2211db6b26643d1b80c2ae6 Primary data file for dataset ID 780386

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1638834

Deployments

None available yet

Instruments

CHN Elemental Analyzer

Supplied Name: Elemental analyzer (CE-440, Exeter Analytical, North Chelmsford, MA)

Supplied Description:

Used for elemental analyses.

Instrument Type

Generic Name: CHN Elemental Analyzer

Acronym: CHN_EA

Generic Description:

A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.

Microscope - Optical

Supplied Name: Eclipse E800 microscope (Nikon, Tokyo, Japan)

Supplied Description:

Used t measure cell volume.

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Species	Species	unitless	species
Strain	Strain	unitless	sample_descrip
GenBank	GenBank Accession Number associated with each strain	unitless	accession_number
Collection_date	Date of collection from the environment; formatted as yyyy-mm-dd	unitless	date
Isolation_Lat	Latitude of strain isolation; north is positive	degrees	lat
Isolation_Lon	Longitude of strain isolation; east is positive	degrees	lon
Isolation_Temperature	Sea surface temperature (SST) at time and position of isolation	degrees C	SST
Temperature	Experimental temperature at which measurements were recorded	degrees C	temp_incub
meanC	Mean carbon per cell	picomol carbon per cell	C
seC	Standard error of carbon per cell	picomol carbon per cell	C
meanN	Mean nitrogen per cell	picomol nitrogen per cell	N
seN	Standard error of nitrogen per cell	picomol nitrogen per cell	N
meanCN	Average molar ratio of carbon to nitrogen per cell	unitless	C_to_N
seCN	Standard error of molar carbon to nitrogen per cell	unitless	C_to_N
meanV	Mean cell volume	cubic micrometers (um3)	volume
seV	Cell volume standard error	cubic micrometers (um3)	volume
mean_cd	Mean carbon density per cell	femtomol/cubic micrometers (fmol um-3)	C
se_cd	Carbon density per cell standard error	femtomol/cubic micrometers (fmol um-3)	C
mean_nd	Mean nitrogen density per cell	femtomol/cubic micrometers (fmol um-3)	N
se_nd	Nitrogen density per cell standard error	femtomol/cubic micrometers (fmol um-3)	N