Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Parameters
• Instruments
• Deployments
• Project Information
• Funding

Summary of samples collected by the Hydrothermal Organic Geochemistry (HOG) sampler on AT-4201 with ROV Jason, on dives J2_1107 through J2_1111, during R/V Atlantic cruise AT42-01, September 9 - October 1, 2018.

Fluid samples were collected into either 2 L or 11 L kynar bags that were acid washed (10% HCl soak overnight, followed by through rinsing, overnight Milli-Q soak, and further rinsing). Sample for geochemistry were collected unammended. Samples for microbiology were pre-spiked with 13C-labeled formate, bicarbonate, or methane, or with preservative. Additional samples were collected by filtering fluids in situ through sterivex filters.

Average T refers to the average of the fluid temperature measured while the sample was being collected. Temperatures were measured with NKE Instrumentation Temperature sensors (P/N S2T6000-Ti-DH; P/N 60-07-326-002). "N.D." is no data.

BCO-DMO Processing:
- created Sample_Descrip column;
- re-formatted date to yyyy-mm-dd;
- added start and end date/time columns in ISO 8601 format;
- replaced commas with semi-colons in the Comment column.

NSF Award Abstract:
The vast majority of deep seafloor sediments are inhabited by microbial communities that survive under extreme energy limitation, with apparent generation times of centuries to millennia. Hydrothermal systems are a stark contrast to these energy-starved environments and may represent important, high-activity, 'population centers' in the oceanic subsurface. When rocks from the Earth's mantle are uplifted and exposed to water, the resulting reactions lead to acidic fluids with high concentrations of hydrogen. Under certain circumstances, small organic molecules such as methane can also form in the absence of biology. These compounds can provide energy to subseafloor microbial communities and, given the ubiquity of mantle rocks, such reactions may fuel a significant proportion of the active subsurface biosphere. The current project will characterize the microbial communities inhabiting an iconic example of this type of system, the Lost City Hydrothermal Field, using a remotely operated vehicle. The ghostly spires of Lost City are highly telegenic and have been featured in professional documentaries. The high definition underwater video footage collected during the expedition will provide the raw material for an 8 week educational training program in digital media focused on kindergarten through 12th grade high school students and undergraduate students. The resulting short documentaries will be published on YouTube and the Utah Education Network.

Mantle rocks comprise significant portions of the seafloor, and microbial communities hosted within them may be important mediators of carbon and energy exchange between the deep Earth and the surface biosphere. Upon tectonic uplift and exposure to water, the serpentinization of these materials releases potential energy in the form of hydrogen, methane, and heat, and further reaction of these products can sustain the abiogenic synthesis of small organic molecules. Recent studies have highlighted, however, the lack of alkalithermophiles that are capable of survival at the high pH (9-11) and elevated temperatures found in these systems. The almost complete lack of carbon dioxide (CO2) represents a second, and possibly more significant, limitation to growth. To better understand the extent of the serpentinite subsurface, this project will address the question: What limits biological activity in the serpentinite subsurface? Specifically, the proposed work will test the hypotheses: (1) microbial diversity spans a wider range of temperature-pH conditions than currently recognized and (2) the scarcity of CO2 is a key biological limitation to serpentinization-driven ecosystems that can be overcome by the metabolic activity of one or a few foundation species. These hypotheses will be tested during a 20 day (10 days on site) expedition to the Lost City Hydrothermal Field, focusing on fluids as windows to the subsurface biosphere. The sampling approach will capitalize on the differences in temperature, carbon availability, and microbial activity across the field. The analytical approach will integrate multidisciplinary techniques performed on replicate subsamples and feature the application of next-generation sequencing technologies to these marine serpentinizing fluids for the first time. This study will generate extensive sequence data from environmental DNA, environmental mRNA, and single-cell genomes, allowing us to identify the in situ expression of metabolic pathways and the genomics of active single cells. These efforts will be closely linked with a thorough characterization of carbon in these fluids that will focus on identifying available substrates (e.g. methane, CO2, organic acids) and on characterizing biomarkers that reflect specific metabolic pathways (e.g. lipids, amino acids).