Dataset: Varadero Transplant Mapping

Name: Metadata information for all samples taken from coral fragments used in a transplant experiment conducted at the Varadero Reef from 2016-2017
Published: 2020-02-05
Keywords: oceans

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Spatial Extent: N:10.30647222 E:-75.57694444 S:10.18669444 W:-75.74527778

Temporal Extent: 2016-10-14 - 2017-05-03

Project:

RAPID: Coral robustness: lessons from an "improbable" reef (Varadero Reef)

Principal Investigator:

Mónica Medina (Pennsylvania State University, PSU)

Co-Principal Investigator:

Roberto Iglesias-Prieto (Pennsylvania State University, PSU)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2020-02-05

Restricted:

Validated:

Current State:

Data not available

Metadata information for all samples taken from coral fragments used in a transplant experiment conducted at the Varadero Reef from 2016-2017

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Description

This dataset contains metadata information for all samples taken from coral fragments used in a transplant experiment. These samples were collected at two time points (pre-transplant and post-trasplant 6 months later) from Orbicella faveolata fragments originating from Varadero (3.5m depth) and Rosario (12m depth). Samples were also transplanted to a site within Cartagena Bay at 3m depth. The file contains one spreadsheet with information on dates of sampling, depths, temperature, fragment origin, and fragment status. This metadata file was used to organize and analyze sample 16S sequences.

Methods & Sampling

The Varadero Reef is located south-west of the Cartagena Bay close to the southern strait that connects the Bay to the Caribbean Sea in Colombia (10°18'23.3"N, 75°35'08.0"W). The Bay is a receiving estuary from the Magdalena River through the Canal del Dique, a man-made channel whose construction and operation dates back almost a century. Three study sites were considered in order to evaluate the role of the local light environment associated to the Canal del Dique plume on the photosynthetic performance of corals from Varadero: 1) Varadero reef at 3.5m depth (10°18'23.3"N, 75°35'08.0"W), 2) Punta Brava reef at 12m depth (10°11'12.1"N, 75°44'43.0"W), and 3) Cartagena Bay at 3m depth (10°18'5.80"N, 75°34'37.10"W).

Samples were collected using hammers and chisels as well as corers. Water samples were collected by passing approximately 1 L of water through a 0.2 micron Sterivex filter.

A full metadata sheet was filled out for every fragment that was collected, underwater using underwater paper, at the time of collection. This includes information on the parent colony of the fragment, fragment tag/ID, depth, temperature, visibility, time of sampling, and health state. Data recorded on under water paper was scanned and then copied into Excel. This Excel file was used as a metadata file to process/analyze 16S sequence data from the samples listed. See related dataset: https://www.bco-dmo.org/dataset/789365

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1642311

Deployments

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Instruments

Manual Biota Sampler

Supplied Name:

Supplied Description:

Samples were collected using hammers and chisels as well as corers.

Instrument Type

Generic Name: Manual Biota Sampler

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/90/

Generic Description:

"Manual Biota Sampler" indicates that a sample was collected in situ by a person, possibly using a hand-held collection device such as a jar, a net, or their hands. This term could also refer to a simple tool like a hammer, saw, or other hand-held tool.

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Dataset: Varadero Transplant Mapping