Dataset: Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 – cell abundance and size by flow cytometry

ValidatedRelease Date:2020-08-31Final no updates expectedDOI: 10.26008/1912/bco-dmo.807327.1Version 1 (2020-04-01)Dataset Type:experimental

Principal Investigator: Uta Passow (University of California-Santa Barbara)

Co-Principal Investigator: Edward Laws (Louisiana State University College of the Coast and Environment)

Scientist, Contact: Nigel D'Souza (University of California-Santa Barbara)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)

Project: Collaborative Research: Effects of multiple stressors on Marine Phytoplankton (Stressors on Marine Phytoplankton)

Abstract

The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacteria Synechococcus elongatus CCMP1629 in a multifactorial design. This dataset contains measurements of cell abundances and cell size expressed as forward scatter (FSC) as well as in equivalent spherical diameter (ESD) in um.

Cite Dataset

The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacteria Synechococcus elongatus CCMP1629 in a multifactorial design. This dataset contains measurements of cell abundances and cell size expressed as forward scatter (FSC) as well as in equivalent spherical diameter (ESD) in um.

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Methods
Dickson, A. G. (1993). The measurement of sea water pH. Marine Chemistry, 44(2-4), 131–142. doi:10.1016/0304-4203(93)90198-w
Methods
Dickson, A. G., & Millero, F. J. (1987). A comparison of the equilibrium constants for the dissociation of carbonic acid in seawater media. Deep Sea Research Part A. Oceanographic Research Papers, 34(10), 1733–1743. doi:10.1016/0198-0149(87)90021-5
Methods
Guillard, R. R. L. (1975). Culture of Phytoplankton for Feeding Marine Invertebrates. Culture of Marine Invertebrate Animals, 29–60. doi:10.1007/978-1-4615-8714-9_3
Methods
Kester, D. R., Duedall, I. W., Connors, D. N., & Pytkowicz, R. M. (1967). Preparation of Artificial Seawater 1. Limnology and Oceanography, 12(1), 176–179. doi:10.4319/lo.1967.12.1.0176
Methods
Mehrbach, C., Culberson, C. H., Hawley, J. E., & Pytkowicx, R. M. (1973). Measurement of the apparent dissociation constants of carbonic acid in seawater at atmospheric pressure. Limnology and Oceanography, 18(6), 897–907. doi:10.4319/lo.1973.18.6.0897

Passow, U., Laws, E., D'Souza, N. (2020) Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 – cell abundance and size by flow cytometry. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-04-01 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.807327.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation