Dataset: Unprocessed holographic data of cryopeg fluids viewed at sub-micron resolution from Alaskan Arctic Coast Permafrost Tunnel and landfast sea ice from May 2017

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.817454.1Version 1 (2020-07-01)Dataset Type:Other Field Results

Principal Investigator: Jody W. Deming (University of Washington)

BCO-DMO Data Manager: Mathew Biddle (Woods Hole Oceanographic Institution)


Program: Marine Microbiology Initiative (MMI)

Project: Understanding How Virus Infection Affects Gene Flow and Microbial Evolution in Extreme Polar Environments (Arctic Subzero Brines)


Abstract

Unprocessed (raw) holographic data of cryopeg and sea ice brines viewed using a digital holographic microscope at sub-micron resolution. Samples collected from Alaskan Arctic Coast Permafrost Tunnel and landfast sea ice near Utqiagvik in May 2017.

Brine samples were collected from both sea ice and cryopeg near Utqiaġvik, Alaska, USA. Snow and ice thickness along with sackhole core depth information are available for sea ice samples. Bacterial and viral abundances along with temperature, pH, salinity, inorganic nutrients, organic nutrients, EPS, and water isotopes were measured for select samples.


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Methods

Cooper, Z. S., Rapp, J. Z., Carpenter, S. D., Iwahana, G., Eicken, H., & Deming, J. W. (2019). Distinctive microbial communities in subzero hypersaline brines from Arctic coastal sea ice and rarely sampled cryopegs. FEMS Microbiology Ecology, 95(12). doi:10.1093/femsec/fiz166
Methods

Lindensmith, C. A., Rider, S., Bedrossian, M., Wallace, J. K., Serabyn, E., Showalter, G. M., … Nadeau, J. L. (2016). A Submersible, Off-Axis Holographic Microscope for Detection of Microbial Motility and Morphology in Aqueous and Icy Environments. PLOS ONE, 11(1), e0147700. doi:10.1371/journal.pone.0147700
Methods

Wallace, J. K., Rider, S., Serabyn, E., Kühn, J., Liewer, K., Deming, J., … Nadeau, J. (2015). Robust, compact implementation of an off-axis digital holographic microscope. Optics Express, 23(13), 17367. doi:10.1364/oe.23.017367