Dataset: Primary production from the San Pedro Ocean Time-series (SPOT) measured between 2014 to 2016

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.821539.1Version 1 (2020-08-20)Dataset Type:Other Field Results

Principal Investigator: Alyson E. Santoro (University of California-Santa Barbara)

Co-Principal Investigator: Douglas G. Capone (University of Southern California)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Project: Collaborative Research: New Approaches to New Production (N-SPOT)


Abstract

Primary production from the San Pedro Ocean Time-series (SPOT) measured between 2014 to 2016. Water samples were collected using a Niskin bottle rosette equipped with a CTD. Rates of primary production were measured by quantifying the rate of CO2 fixation via H¹³CO₃ uptake.

From 2014 to 2015, water samples were collected using a 12 x 12 L Niskin bottle rosette equipped with a conductivity, temperature, and density (CTD) instrument package (SBE 9plus, Sea-Bird Electronics, Bellevue, Washington, USA), including dissolved oxygen (SBE 43) and photosynthetically available radiation (PAR, LI-COR, Biospherical Instruments Inc., San Diego, California, USA) sensors. Due to CTD failure, samples collected in 2015 and 2016 were collected primarily using manually triggered Go-Flo bottles and depths were chosen primarily using a profiling natural fluorometer (PNF) system as well as secchi disk.

Rates of primary production were measured by quantifying the rate of CO2 fixation via H¹³CO₃ uptake. Samples were analyzed using an IsoPrime continuous flow isotope ratio mass spectrometer. Quadruplicate samples were collected and amended to a final concentration of ~25 µmol L-1 of H¹³CO₃. Incubations were carried out for 24 hrs at ambient temperature and light.


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