Dataset: Series 1B-3: Multiple stressor experiments on T. pseudonana (CCMP1335) – computed data from the LC3 protocol for samples at 4 temperatures, 15-26C

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.829009.1Version 1 (2020-11-12)Dataset Type:experimental

Principal Investigator: Uta Passow (University of California-Santa Barbara)

Co-Principal Investigator: Edward Laws (Louisiana State University College of the Coast and Environment)

Scientist, Contact: Julia Sweet (University of California-Santa Barbara)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)

Project: Collaborative Research: Effects of multiple stressors on Marine Phytoplankton (Stressors on Marine Phytoplankton)

Abstract

Four follow-up experiments on the combined effect of light and temperature changes on the growth rate (mu) and photophysiology of Thalassiosira pseudonana CCMP 1335 were conducted to supplement / repeat series 1A experiments. This was necessary because doubt existed regarding the growth during 1A experiments. 1A experiments were conducted in artificial seawater. 1B experiments were conducted in artificial seawater supplemented with 5% sterilized seawater. The experiments were designed to test th...

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The raw fluorescence data can be found under the Data Files section as an Excel file and as individual .csv files.

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Methods
Dickson, A. G. (1993). The measurement of sea water pH. Marine Chemistry, 44(2-4), 131–142. doi:10.1016/0304-4203(93)90198-w
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Dickson, A. G., & Millero, F. J. (1987). A comparison of the equilibrium constants for the dissociation of carbonic acid in seawater media. Deep Sea Research Part A. Oceanographic Research Papers, 34(10), 1733–1743. doi:10.1016/0198-0149(87)90021-5
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Guillard, R. R. L. (1975). Culture of Phytoplankton for Feeding Marine Invertebrates. Culture of Marine Invertebrate Animals, 29–60. doi:10.1007/978-1-4615-8714-9_3
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Kester, D. R., Duedall, I. W., Connors, D. N., & Pytkowicz, R. M. (1967). Preparation of Artificial Seawater 1. Limnology and Oceanography, 12(1), 176–179. doi:10.4319/lo.1967.12.1.0176
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Mehrbach, C., Culberson, C. H., Hawley, J. E., & Pytkowicx, R. M. (1973). Measurement of the apparent dissociation constants of carbonic acid in seawater at atmospheric pressure. Limnology and Oceanography, 18(6), 897–907. doi:10.4319/lo.1973.18.6.0897

Passow, U., Laws, E., Sweet, J. (2020) Series 1B-3: Multiple stressor experiments on T. pseudonana (CCMP1335) – computed data from the LC3 protocol for samples at 4 temperatures, 15-26C. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-11-12 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.829009.1 [access date]

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