Dataset: Depth profile data from R/V New Horizons NH1418 in the tropical Pacific from Sept-Oct. 2014

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.829895.1Version 1 (2020-11-19)Dataset Type:Cruise Results

Principal Investigator: Michael W. Lomas (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator: Adam Martiny (University of California-Irvine)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Program: Dimensions of Biodiversity (Dimensions of Biodiversity)

Program: Ocean Carbon and Biogeochemistry (OCB)

Project: Biological Controls on the Ocean C:N:P ratios (Biological C:N:P ratios)


Abstract

Depth profile data including CTD, oxygen, chlorophyll, light, nutrients, microbe abundances, and DNA sample log from R/V New Horizons NH1418 in the tropical Pacific from Sept-Oct. 2014.

Temperature, salinity, oxygen concentration and saturation, and PAR were measured using a Sea-Bird SBE-911+ CTD platform equipped on the rosette deployment system. Fluorescence was measured via the rosette system using a WetLabs ECO AFL/FL platform.

Samples for NO3-/NO2- and NO2- were gravity filtered through 0.8 µm Nucleopore polycarbonate filters using acid cleaned in-line polycarbonate filter holders, then frozen (-20oC) in HDPE bottles until analysis on an Alpkem Flow Solution IV (Dore et al. 1996).

Soluble reactive phosphorus was measured after preparation via the magnesium-induced coprecipitation method (Karl and Tien 1992; Lomas et al. 2010).

Particulate organic carbon (POC), nitrogen (PON), and phosphorus samples were filtered on precombusted Whatman GF/F filters and frozen until analysis. After thawing, POC/PON filters were allowed to dry overnight at 65◦C before being packed into a 30 mm tin capsule (CE Elantech, Lakewood, New Jersey). Samples were then analyzed for C and N content on a FlashEA 1112 nitrogen and carbon analyzer (Thermo Scientific, Waltham, Massachusetts). POC and PON concentrations were calibrated using known quantities of atropine.  Particulate organic phosphorus samples (POP) are analyzed using a ash-hydrolysis method (Lomas et al., 2010)

For chlorophyll, ~ 250–500 mL seawater was filtered onto 25-mm Ahlstrom glass fiber filters (nominal pore size 0.7 μm) under low pressure (15 kpa), and frozen immediately at −80_C. Samples were extracted in 90% acetone in the dark for 14–18 h at −20_C and quantified on a Turner 10-AU fluorometer using the acidification method (Parsons et al. 1984).

For cell counts, samples of whole seawater were collected in 2-mL centrifuge tubes, fixed with freshly made 0.2 -μm-filtered paraformaldehyde (0.5% v/v final concentration) for 1 h at 5_C in the dark, and counted on a FACSJazz or Influx flow cytometer (BD, Franklin Lakes, NJ, U.S.A.) utilizing a 200 mW 488 nm laser, with detectors for forward scatter, side scatter, 530 nm, and 692 nm. Prochlorococcus populations were discriminated based on forward scatter and red fluorescence, and a gate in orange (585 nm) discriminated for Synechococcus. Picoeukaryotic phytoplankton were all the red auto fluorescing cells that did not fit the Cyanobacteria gating scheme with a cell size below 2 – 3 μm.

See https://www.rvdata.us/search/cruise/NH1418 for further details.

For published methodologies please see the Related Publications section.


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Related Publications

Results

Garcia, C.A., Hagstrom, G.I., Larkin, A.A., Ustick, L.J., Levin, S.A., Lomas, M.W., & Martiny AC. (2020). Linking regional shifts in microbial genome adaptation with surface ocean biogeochemistry. Philosophical Transactions of the Royal Society B, 375, 20190254. doi: 10.1098/rstb.2019.0254
Results

Kent, A. G., Baer, S. E., Mouginot, C., Huang, J. S., Larkin, A. A., Lomas, M. W., & Martiny, A. C. (2018). Parallel phylogeography of Prochlorococcus and Synechococcus. The ISME Journal, 13(2), 430–441. doi:10.1038/s41396-018-0287-6
Methods

Baer, S. E., Lomas, M. W., Terpis, K. X., Mouginot, C., & Martiny, A. C. (2017). Stoichiometry of Prochlorococcus, Synechococcus, and small eukaryotic populations in the western North Atlantic Ocean. Environmental Microbiology, 19(4), 1568–1583. doi:10.1111/1462-2920.13672
Methods

Cetinić, I., Poulton, N., & Slade, W. H. (2016). Characterizing the phytoplankton soup: pump and plumbing effects on the particle assemblage in underway optical seawater systems. Optics Express, 24(18), 20703. doi:10.1364/oe.24.020703
Methods

Dore, J., Houlihan, T., Hebel, D., Tien, G., Tupas, L., and Karl, D. (1996) Freezing as a method of sample preservation for the analysis of dissolved inorganic nutrients in seawater. Marine Chemistry 53, 173-185.