Dataset: Acer Fecundity Oocyte Size

Name: Fecundity and oocyte sizes of Acropora cervicornis genotypes measured July 2020 at Mote Marine Lab
Published: 2022-03-23
Keywords: Coral, Fecundity, oocyte, reproduction, biota, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.843067.1

Spatial Extent: N:24.56257 E:-81.40009 S:24.45782 W:-81.88595

Temporal Extent: 2020-07-25

Project:

CAREER: Applying phenotypic variability to identify resilient Acropora cervicornis genotypes in the Florida Keys (Resilient Acerv)

Principal Investigator:

Erinn M. Muller (Mote Marine Laboratory, Mote)

Co-Principal Investigator:

Hanna Koch (Mote Marine Laboratory, Mote)

Scientist:

Erich Bartels (Mote Marine Laboratory, Mote)

Student:

Yuen Azu (Mote Marine Laboratory, Mote)

BCO-DMO Data Manager:

Dana Stuart Gerlach (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2022-03-23

Restricted:

Release Date:

2022-12-31

Validated:

Yes

Current State:

Final no updates expected

Fecundity and oocyte sizes of Acropora cervicornis genotypes measured July 2020 at Mote Marine Lab

Abstract:

Primary fecundity was assessed for Acropora cervicornis corals with known disease susceptibility. This dataset presents information on oocyte sizes from dissections of coral polyps from five adult colonies containing 12 genets held in Mote Marine Lab’s spawning nurseries.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2022-03-23	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.843067.1	2022-03-24

Description

This dataset is part of a larger study of Acropora cervicornis (staghorn) corals studied at Mote Marine Lab’s Elizabeth Moore International Center for Coral Reef Research and Restoration. We present dissection information on the sizes of oocytes measured from five randomly chosen oocytes for each of five replicate polpys from three fragments of five colonies containing 12 different genets of Acropora cervicornis held in Mote Marine Laboratory's Sand Key spawing nursery.

The project's additional analyses are listed here, and links to other data from this study can be found in the Related Datasets section below.

Analyses undertaken include:

Total Population (Colony size dataset)
Morphometric Assessment (Colony size dataset)
Primary Fecundity Analysis (Colony size dataset's population subset, Polyps dataset)
Dissections (this dataset; plus Oocyte number dataset)
Secondary Fecundity Analysis (Gamete bundle dataset)

See Related Datasets section below for links to above mentioned datasets.

Methods & Sampling

Sampling of Acropora cervicornis coral genotypes took place at Mote Marine Lab's spawning nurseries at Sand Key (24.45782, -81.88595) and Looe Key (24.56257, -81.40009). Primary fecundity analysis involved sampling corals with known disease susceptibility and/or resistance, measuring their size, and counting and analyzing their oocytes.

Sampling for Primary Fecundity Analysis:
On July 3, 2020, from 5 colonies of every genet, 3 linear branches (~10 cm in length) were sampled using bone cutters from the central portion of each colony (N=180 branches). Using a ruler, the number of polyps per one square centimeter was recorded from every branch near the base of the fragment (Polyps per Area dataset, https://www.bco-dmo.org/dataset/868308). The top ~2 cm (sterile zone) of every branch was removed before placing into a 50 mL falcon tube with 10% formalin to fix tissues. After 2 days, the formalin solutions were replaced with a 5% HCl solution, with every branch and tube triple rinsed with DI water in between to remove excess formalin. After 3 days, the 5% HCl solution in every tube was replaced with 10% HCl and subsequently refreshed every 2-3 days until branches were completely decalcified. Once decalcified, samples were triple rinsed in DI water and returned to their tubes with 70% EtOH for storage until dissection.

Dissections (this dataset):
Under a dissecting microscope, every fragment was dissected using scalpel and forceps to haphazardly select 5 polyps per fragment (N = 900 polyps) to count the total number of oocytes within each polyp (Oocyte Number Dataset, https://www.bco-dmo.org/dataset/867314). From those, 5 oocytes were randomly selected to measure their size under a compound microscope using an ocular micrometer to record the maximum diameter (length, d1) and its perpendicular diameter (width, d2). The volume of oocytes was calculated using the formula for a prolate ellipsoid: V= (4/3)*pi*(d1/2)*(d2/2)^2

Calibration factor: Oocytes were measured using a 10x ocular micrometer and 4x objective (total 40X) and a calibration factor was applied using the formula: V= (4/3)*pi*((d1/2)*250)*((d2/2)*250)^2 and converted to the units, mm3, for final values.

Problem report:
Genet 31: In the time between when the parental colonies were morphometrically assessed/sampled in the Sand Key nursery for the primary fecundity analysis and brought into the lab for spawning/sampled for the secondary fecundity analysis, the entire tree for genet 31 snapped off from its anchor and went missing. As such, 5 colonies of genet 31 were brought in from a different spawning nursery and location (Looe Key Nursery: 24.56257, -81.40009). Thus, the fecundity data obtained from the fragments and gamete bundles come from different subpopulations (fragments from Sand Key and gamete bundles from Looe Key).

Data Processing Description

Data were compiled into Excel (Microsoft Office) and analyzed using R version 4.0.3 (2020-10-10) -- "Bunny-Wunnies Freak Out". Nonparametric statistical and correlation analyses were conducted.

BCO-DMO Processing:
- separated Latitude and Longitude into separate columns
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions

Data Files

File
oocyte_size.csv (Comma Separated Values (.csv), 400.78 KB) MD5:1e7aca6e4e1673da81866cf18bc780db Primary data file for dataset ID 843067

Supplemental Files

File
Disease_Susceptibility_Table filename: Disease_Susceptibility_Table.pdf (Portable Document Format (.pdf), 64.24 KB) MD5:b155930985bd0c9e954a1f0eeacc78a1 Acropora cervicornis genotypes and susceptibility to white-band disease

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1452538

Deployments

None available yet

Instruments

bone cutter

Supplied Name: Calcutta metal pliers

Supplied Description:

Each ramet was cut from the donor colony using metal pliers (Calcutta bone cutters).

Instrument Type

Generic Name: bone cutter

Generic Description:

A bone cutter is a surgical instrument used to cut bones or coral fragments.

Microscope - Optical

Supplied Name: AmScope compound microscope with ocular micrometer

Supplied Description:

Oocyte size was measured under a compound microscope using an ocular micrometer to record the maximum diameter (length, d1) and its perpendicular diameter (width, d2).

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Microscope - Optical

Supplied Name: articulating dissecting microscope

Supplied Description:

Under a dissecting microscope, every fragment was dissected using a scalpel and forceps and to count the total number of oocytes within each polyp.

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

scalpel

Supplied Description:

Every coral fragment was dissected using a scalpel and forceps

Instrument Type

Generic Name: scalpel

Generic Description:

A scalpel, or lancet, or bistoury, is a small and extremely sharp bladed instrument used for dissection and surgery.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Location	Location of coral sampling site	unitless	site
Latitude	Latitude of spawning nursery	decimal degrees	lat
Longitude	Longitude of spawning nursery	decimal degrees	lon
Date_measured	Date when measurements were taken (local time) Format: %Y-%m-%d	unitless	date
Genotype	Mote Marine Lab genet that was sampled (1, 3, 7, 13, 31, 34, 41, 44, 47, 50, 62, 63)	unitless	sample_type
Phenotype	Phenotype that was sampled (S = white band disease susceptible, R = white band disease resistant)	unitless	sample_type
Replicate_Colony	Which of the five replicate colonies of the genet was sampled for analysis	unitless	replicate
Replicate_Fragment	Which of the three replicate fragments per replicate colony was sampled for analysis	unitless	replicate
Replicate_Polyp	Which of the five polyps was randomly chosen to be dissected and counted for number of oocytes	unitless	replicate
Replicate_Oocyte	Which of the 5 oocytes per polyp that was randomly chosen for size measurement	unitless	sample
Oocyte_Length_d1	Length of the oocyte’s longest axis	micrometers	length
Oocyte_Width_d2	Width perpendicular to the oocyte's longest axis	micrometers	width
Oocyte_Volume_micron	Oocyte volume calculated using a prolate ellipsoid (V= (4/3)pi(d1/2)*(d2/2)^2)	cubic micrometers	sample_volume
Oocyte_Vol_corrected	Oocyte volume with applied calibration factor using 10x ocular micrometer and 4x objective	cubic micrometers	sample_volume
Oocyte_Volume_mm3	Oocyte size converted to more commonly used unit of volume	cubic millimeters	sample_volume

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Database

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Dataset: Acer Fecundity Oocyte Size