Dataset: An investigation of Symbiodiniaceae communities via ITS-2 rDNA amplicon sequencing in Acropora millepora corals from the Great Barrier Reef following exposure to stressors in October 2014

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.844431.1Version 1 (2021-03-31)Dataset Type:Other Field Results

Principal Investigator: Adrienne M.S. Correa (Rice University)

Student: Lauren I. Howe-Kerr (Rice University)

BCO-DMO Data Manager: Dana Stuart Gerlach (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Taylor Heyl (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Viral Reefscapes: The Role of Viruses in Coral Reef Health, Disease, and Biogeochemical Cycling (Moorea Virus Project)


Abstract

Symbiodiniaceae communities were investigated at three locations on the Great Barrier Reef in October 2014. Acropora millepora samples from Davies Reef lagoon (18°30′3.96′′S, 147°22′48′′E), Rib Reef (18°28′53.4′′S, 146°52′24.96′′E), and Pandora Island (18°48′45′′S, 146°25′59.16′′E), were exposed to various stressors including pCO2, heat, bacteria, all of these, or none of these (control). This dataset lists accessions and collection information for ITS-2 rDNA amplicon data that are available a...

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Symbiodiniaceae communities from Acropora millepora corals were investigated at three locations on the Great Barrier Reef between October 1st and 8th, 2014.  Samples from Davies Reef lagoon, Rib Reef, and Pandora Island were collected and snap frozen in liquid nitrogen and stored at -20℃ until further processing.  DNA was extracted using Wayne's Method (Wilson et al., 2002; Lundgren et al., 2013) from tissue slurry prepared following the method of Wright et al. (2019).

This dataset lists accessions and collection information for ITS-2 rDNA amplicon data from Acropora millepora corals following exposure to various stressors. Stressors included pCO2, heat, bacteria, all of these, or none of these (control).  ITS-2 rDNA Symbiodiniaceae community libraries were prepared and PE 300bp reads were generated using Illumina MiSeq platform. BioSamples and SRA accessions are available at the National Center for Biotechnology Information (NCBI) under BioProject PRJNA596498 (https://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA596498)


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Results

Howe‐Kerr, L. I., Bachelot, B., Wright, R. M., Kenkel, C. D., Bay, L. K., & Correa, A. M. S. (2020). Symbiont community diversity is more variable in corals that respond poorly to stress. Global Change Biology, 26(4), 2220–2234. doi:10.1111/gcb.14999
Methods

Cunning, R., Gates, R. D., & Edmunds, P. J. (2017). Using high-throughput sequencing of ITS2 to describe Symbiodinium metacommunities in St. John, US Virgin Islands. PeerJ, 5, e3472. doi:10.7717/peerj.3472
Methods

Frøslev, T. G., Kjøller, R., Bruun, H. H., Ejrnæs, R., Brunbjerg, A. K., Pietroni, C., & Hansen, A. J. (2017). Algorithm for post-clustering curation of DNA amplicon data yields reliable biodiversity estimates. Nature Communications, 8(1). doi:10.1038/s41467-017-01312-x
Methods

Illumina. 16S Metagenomic Sequencing Library Preparation: Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System. (2013). [cited 2018 Oct 18]; Available from: https://support.illumina.com/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf
Methods

Lundgren, P., Vera, J. C., Peplow, L., Manel, S., & van Oppen, M. J. (2013). Genotype – environment correlations in corals from the Great Barrier Reef. BMC Genetics, 14(1), 9. doi:10.1186/1471-2156-14-9