Dataset: March Incubation Phytoplankton Elemental Composition

Name: Elemental composition of phytoplankton communities from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI.
Published: 2021-04-26
Keywords: Multiple drivers, environmental filtering, phytoplankton physiology, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.848587.1

Spatial Extent: N:41.57 E:-71.39 S:41.57 W:-71.39

Temporal Extent: 2017-03-20

Project:

Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients (Phytoplankton Community Responses)

Program:

Dimensions of Biodiversity (Dimensions of Biodiversity)

Principal Investigator:

Stephanie I. Anderson (University of Rhode Island, URI-GSO)

Co-Principal Investigator:

Gayantonia Franzè (Institute of Marine Research (Bergen, Norway), IMR)

David A. Hutchins (University of Southern California, USC)

Joshua D. Kling (University of California-Berkeley, UC Berkeley)

Colin T. Kremer (University of California-Los Angeles, UCLA)

Elena Litchman (Michigan State University, MSU)

Susanne Menden-Deuer (University of Rhode Island, URI-GSO)

Tatiana A. Rynearson (University of Rhode Island, URI-GSO)

Paul Wilburn (Michigan State University, MSU)

Contact:

Stephanie I. Anderson (University of Rhode Island, URI-GSO)

BCO-DMO Data Manager:

Taylor Heyl (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-04-26

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Elemental composition of phytoplankton communities from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI.

Abstract:

This dataset reports the elemental composition of phytoplankton communities from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI. These data were assessed in Anderson et al. The Interactive Effects of Temperature and Nutrients on a Spring Phytoplankton Community (in prep).

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2021-04-26	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.848587.1	2021-04-28

Methods & Sampling

The elemental composition of phytoplankton communities was analyzed via multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI. Water was incubated in triplicate at -0.5ºC, 2.6ºC, and 6ºC for 10 days. At each temperature, treatments included both nutrient amendments (N, P, Si addition) and controls (no macronutrients added).

At the onset and conclusion of the incubation for all treatments, and additionally at each dilution time point for 2.6 and 6ºC amended treatments, each biological incubation replicate was assessed for particulate organic carbon (POC) and nitrogen (PON), and biogenic silica (BSi) content. POC and PON were evaluated in triplicate by harvesting cells onto 25 millimeter (mm) GF/F filters which had been pre-combusted at 450ºC for 24 hours. Filters were then analyzed on a Costech Elemental Combustion System (Costech Analytical Technologies Inc.). BSi content was assessed by filtering cells in triplicate onto 2 micrometer (µm) polycarbonate filters and analyzing them on a Barnstead Turner SP-830 spectrophotometer, following the methods of Strickland and Parsons (1972). Additionally, nutrient analyses of ammonium, phosphate, silicate, and nitrite/nitrate (total inorganic nitrogen) were evaluated using a Lachat Quikchem 8500 analyzer (Hach) at the University of Rhode Island Marine Science Research Facility.

For complete methodology, see “The Interactive Effects of Temperature and Nutrients on a Spring Phytoplankton Community” (Anderson et al, in prep).

Note: Due to operator error, one replicate of POC and PON had to be discarded from the 6ºC amended incubation.

Data Processing Description

BCO-DMO processing:
- Adjusted field/parameter names to comply with database requirements
- Missing data identifier ‘NA’ and ‘N/A’ replaced with 'nd' (BCO-DMO's default missing data identifier)
- Added a conventional header with dataset name, PI names, version date
- Columns: uM_Si, uM_C, uM_N, CN, SiC were rounded to the thousandth decimal place

Data Files

File
phyto_elemental_composition.csv (Comma Separated Values (.csv), 1.97 KB) MD5:63e7cc3c60762121c0f113ffa46ed8bc Primary data file for dataset ID 848587

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1638834

Deployments

None available yet

Instruments

Costech International Elemental Combustion System (ECS) 4010

Supplied Name: Costech Elemental Combustion System

Supplied Description:

Instrument Type

Generic Name: Costech International Elemental Combustion System (ECS) 4010

Acronym: Costech ECS 4010

Generic Description:

The ECS 4010 Nitrogen / Protein Analyzer is an elemental combustion analyser for CHNSO elemental analysis and Nitrogen / Protein determination. The GC oven and separation column have a temperature range of 30-110 degC, with control of +/- 0.1 degC.

Flow Injection Analyzer

Supplied Name: Lachat Quikchem 8500 analyzer

Supplied Description:

Lachat Quikchem 8500 analyzer (Hach)

Instrument Type

Generic Name: Flow Injection Analyzer

Acronym: FIA

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB36/

Generic Description:

An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques.

Spectrophotometer

Supplied Name: Turner SP-830 spectrophotometer

Supplied Description:

Turner SP-830 spectrophotometer (Barnstead International)

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Sample	Sample identification number	unitless	sample
Replicate	Biological replicate identification	unitless	replicate
Date	Day of experiment in which sample was collected	days	date
Temperature	Temperature treatment in which incubation was conducted	degrees celsius (°C)	temp
Nutrient	Nutrient treatment in which incubation was conducted	unitless	treatment
uM_Si	Biogenic silica in sample	micromoles per liter (µmol/L)	silica
uM_C	Particulate organic carbon in sample	micromoles per liter (µmol/L)	POC
uM_N	Particulate organic nitrogen in sample	micromoles per liter (µmol/L)	PON
CN	Ratio of carbon to nitrogen in sample	unitless	C_to_N
SiC	Ratio of biogenic silica to carbon in sample	unitless	ratio

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Database

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Dataset: March Incubation Phytoplankton Elemental Composition