Dataset: Images corresponding to some of the samples that underwent 16S and 18S V4 amplicon sequencing of microbial communities in sinking particles collected during R/V Atlantic Explorer cruises AE1718 and AE1809 in 2017 and 2018 at BATS in Bermuda

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.855320.1Version 1 (2021-07-08)Dataset Type:Cruise Results

Principal Investigator: Susanne Neuer (Arizona State University)

Contact: Bianca N. Cruz (Arizona State University)

BCO-DMO Data Manager: Amber D. York (Woods Hole Oceanographic Institution)

Project: Aggregation of Marine Picoplankton (Marine Plankton Aggregation)

Abstract

Images corresponding to some of the samples that underwent 16S and 18S V4 amplicon sequencing of microbial communities in sinking particles collected during R/V Atlantic Explorer cruises AE1718 and AE1809 in 2017 and 2018 at BATS in Bermuda. These data were published in Neuer et al. (2021).

Cite Dataset

Methodology:
Particles to be subjected to prokaryotic and eukaryotic microbial community analyses were imaged whilst embedded within polyacrylamide gels. Images were taken at various magnifications and calibrated using a 1.0 mm object micrometer slide. Particles were subsequently picked, pooled, and subjected to DNA extraction and microbial community analyses as outlined in the Methods text in Neuer et al. (2021).

Although 8 particles were pooled per sample, a limited number of particles were imaged. Magnification metadata is not available for sample PA_150m_Fall.

Related Datasets

IsRelatedTo
Dataset: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA675293
Arizona State University (2020). Prokaryotic and eukaryotic microbial communities within sinking particles from the Bermuda Atlantic Time-series Study (BATS) site. 7-Nov-2020. In: NCBI:BioProject: PRJNA675293. [Internet]. Bethesda, MD: National Library of Medicine (US), National Center for Biotechnology Information; Available from: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA675293.
IsRelatedTo
Dataset: Prokaryotic and eukaryotic abundance in BATS microbial communities
Relationship Description: Data from the same sinking particle samples.
Neuer, S. (2021) Prokaryote and eukaryote abundance in phytodetrital aggregates (PA), fecal aggregates (FA), and the ambient seawater from samples collected during R/V Atlantic Explorer cruises AE1718 and AE1809 in 2017 and 2018 at BATS in Bermuda. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-07-08 doi:10.26008/1912/bco-dmo.855296.1
IsRelatedTo
Dataset: Sinking particle sample information for BATS microbial communities
Relationship Description: Sample information and genetic accession info for the same sinking particle samples.
Neuer, S., Cruz, B. N. (2021) Sample information for 16S and 18S V4 amplicon sequencing of microbial communities in sinking particles and water column samples collected during R/V Atlantic Explorer cruises AE1718 and AE1809 in 2017 and 2018 at BATS in Bermuda. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-11-16 doi:10.26008/1912/bco-dmo.828922.1

Related Publications

Results
Cruz, B. N., Brozak, S., & Neuer, S. (2021). Microscopy and DNA‐based characterization of sinking particles at the Bermuda Atlantic Time‐series Study station point to zooplankton mediation of particle flux. Limnology and Oceanography. doi:10.1002/lno.11910
Software
Schneider, C. A., Rasband, W. S., ... (n.d.). ImageJ. US National Institutes of Health, Bethesda, MD, USA. Available from https://imagej.nih.gov/ij/
Software
ToupTek Photonics (2015). ToupView for ToupCam Camera. Information accessible from http://www.touptek.com/product/showproduct.php?lang=en&id=103

Neuer, S. (2021) Images corresponding to some of the samples that underwent 16S and 18S V4 amplicon sequencing of microbial communities in sinking particles collected during R/V Atlantic Explorer cruises AE1718 and AE1809 in 2017 and 2018 at BATS in Bermuda. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-07-08 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.855320.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation